Cargando…

Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System

Following peripheral nerve injury, dysregulations of certain non-coding microRNAs (miRNAs) occur in Schwann cells. Whether these alterations are the result of local inflammation and/or correlate with perturbations in the expression profile of the protective vasoactive intestinal peptide (VIP)/pituit...

Descripción completa

Detalles Bibliográficos
Autores principales: Musumeci, Giuseppe, Leggio, Gian Marco, Marzagalli, Rubina, Al-Badri, Ghaith, Drago, Filippo, Castorina, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979605/
https://www.ncbi.nlm.nih.gov/pubmed/29587404
http://dx.doi.org/10.3390/ijms19040981
_version_ 1783327736686706688
author Musumeci, Giuseppe
Leggio, Gian Marco
Marzagalli, Rubina
Al-Badri, Ghaith
Drago, Filippo
Castorina, Alessandro
author_facet Musumeci, Giuseppe
Leggio, Gian Marco
Marzagalli, Rubina
Al-Badri, Ghaith
Drago, Filippo
Castorina, Alessandro
author_sort Musumeci, Giuseppe
collection PubMed
description Following peripheral nerve injury, dysregulations of certain non-coding microRNAs (miRNAs) occur in Schwann cells. Whether these alterations are the result of local inflammation and/or correlate with perturbations in the expression profile of the protective vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating polypeptide (PACAP) system is currently unknown. To address these issues, we aimed at profiling the expression of selected miRNAs in the rat RT4 Schwann cell line. Cells exposed to lipopolysaccharide (LPS), to mimic the local inflammatory milieu, were appraised by real-time qPCR, Western blot and ELISAs. We found that upon LPS treatment, levels of pro-inflammatory cytokines (IL-1β, -6, -18, -17A, MCP-1 and TNFα) increased in a time-dependent manner. Unexpectedly, the expression levels of VIP and PACAP were also increased. Conversely, levels of VPAC1 and VPAC2 receptors were reduced. Downregulated miRNAs included miR-181b, -145, -27a, -340 and -132 whereas upregulated ones were miR-21, -206, -146a, -34a, -155, -204 and -29a, respectively. Regression analyses revealed that a subset of the identified miRNAs inversely correlated with the expression of VPAC1 and VPAC2 receptors. In conclusion, these findings identified a novel subset of miRNAs that are dysregulated by immune challenge whose activities might elicit a regulatory function on the VIP/PACAP system.
format Online
Article
Text
id pubmed-5979605
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59796052018-06-10 Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System Musumeci, Giuseppe Leggio, Gian Marco Marzagalli, Rubina Al-Badri, Ghaith Drago, Filippo Castorina, Alessandro Int J Mol Sci Article Following peripheral nerve injury, dysregulations of certain non-coding microRNAs (miRNAs) occur in Schwann cells. Whether these alterations are the result of local inflammation and/or correlate with perturbations in the expression profile of the protective vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating polypeptide (PACAP) system is currently unknown. To address these issues, we aimed at profiling the expression of selected miRNAs in the rat RT4 Schwann cell line. Cells exposed to lipopolysaccharide (LPS), to mimic the local inflammatory milieu, were appraised by real-time qPCR, Western blot and ELISAs. We found that upon LPS treatment, levels of pro-inflammatory cytokines (IL-1β, -6, -18, -17A, MCP-1 and TNFα) increased in a time-dependent manner. Unexpectedly, the expression levels of VIP and PACAP were also increased. Conversely, levels of VPAC1 and VPAC2 receptors were reduced. Downregulated miRNAs included miR-181b, -145, -27a, -340 and -132 whereas upregulated ones were miR-21, -206, -146a, -34a, -155, -204 and -29a, respectively. Regression analyses revealed that a subset of the identified miRNAs inversely correlated with the expression of VPAC1 and VPAC2 receptors. In conclusion, these findings identified a novel subset of miRNAs that are dysregulated by immune challenge whose activities might elicit a regulatory function on the VIP/PACAP system. MDPI 2018-03-25 /pmc/articles/PMC5979605/ /pubmed/29587404 http://dx.doi.org/10.3390/ijms19040981 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Musumeci, Giuseppe
Leggio, Gian Marco
Marzagalli, Rubina
Al-Badri, Ghaith
Drago, Filippo
Castorina, Alessandro
Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title_full Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title_fullStr Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title_full_unstemmed Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title_short Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System
title_sort identification of dysregulated microrna networks in schwann cell-like cultures exposed to immune challenge: potential crosstalk with the protective vip/pacap neuropeptide system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979605/
https://www.ncbi.nlm.nih.gov/pubmed/29587404
http://dx.doi.org/10.3390/ijms19040981
work_keys_str_mv AT musumecigiuseppe identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem
AT leggiogianmarco identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem
AT marzagallirubina identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem
AT albadrighaith identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem
AT dragofilippo identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem
AT castorinaalessandro identificationofdysregulatedmicrornanetworksinschwanncelllikeculturesexposedtoimmunechallengepotentialcrosstalkwiththeprotectivevippacapneuropeptidesystem