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Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse
Ischemia reperfusion injury is one of the major complications responsible for delayed graft function in kidney transplantation. Applications to reduce reperfusion injury are essential due to the widespread use of kidneys from deceased organ donors where the risk for delayed graft function is especia...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979943/ https://www.ncbi.nlm.nih.gov/pubmed/29581529 http://dx.doi.org/10.1038/s41598-018-21463-1 |
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author | Nordling, Sofia Brännström, Johan Carlsson, Fredrik Lu, Bo Salvaris, Evelyn Wanders, Alkwin Buijs, Jos Estrada, Sergio Tolmachev, Vladimir Cowan, Peter J. Lorant, Tomas Magnusson, Peetra U. |
author_facet | Nordling, Sofia Brännström, Johan Carlsson, Fredrik Lu, Bo Salvaris, Evelyn Wanders, Alkwin Buijs, Jos Estrada, Sergio Tolmachev, Vladimir Cowan, Peter J. Lorant, Tomas Magnusson, Peetra U. |
author_sort | Nordling, Sofia |
collection | PubMed |
description | Ischemia reperfusion injury is one of the major complications responsible for delayed graft function in kidney transplantation. Applications to reduce reperfusion injury are essential due to the widespread use of kidneys from deceased organ donors where the risk for delayed graft function is especially prominent. We have recently shown that coating of inflamed or damaged endothelial cells with a unique heparin conjugate reduces thrombosis and leukocyte recruitment. In this study we evaluated the binding capacity of the heparin conjugate to cultured human endothelial cells, to kidneys from brain-dead porcine donors, and to murine kidneys during static cold storage. The heparin conjugate was able to stably bind cultured endothelial cells with high avidity, and to the renal vasculature of explanted kidneys from pigs and mice. Treatment of murine kidneys prior to transplantation reduced platelet deposition and leukocyte infiltration 24 hours post-transplantation, and significantly improved graft function. The present study thus shows the benefits of enhanced protection of the renal vasculature during cold storage, whereby increasing the antithrombotic and anti-adhesive properties of the vascular endothelium yields improved renal function early after transplantation. |
format | Online Article Text |
id | pubmed-5979943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59799432018-06-06 Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse Nordling, Sofia Brännström, Johan Carlsson, Fredrik Lu, Bo Salvaris, Evelyn Wanders, Alkwin Buijs, Jos Estrada, Sergio Tolmachev, Vladimir Cowan, Peter J. Lorant, Tomas Magnusson, Peetra U. Sci Rep Article Ischemia reperfusion injury is one of the major complications responsible for delayed graft function in kidney transplantation. Applications to reduce reperfusion injury are essential due to the widespread use of kidneys from deceased organ donors where the risk for delayed graft function is especially prominent. We have recently shown that coating of inflamed or damaged endothelial cells with a unique heparin conjugate reduces thrombosis and leukocyte recruitment. In this study we evaluated the binding capacity of the heparin conjugate to cultured human endothelial cells, to kidneys from brain-dead porcine donors, and to murine kidneys during static cold storage. The heparin conjugate was able to stably bind cultured endothelial cells with high avidity, and to the renal vasculature of explanted kidneys from pigs and mice. Treatment of murine kidneys prior to transplantation reduced platelet deposition and leukocyte infiltration 24 hours post-transplantation, and significantly improved graft function. The present study thus shows the benefits of enhanced protection of the renal vasculature during cold storage, whereby increasing the antithrombotic and anti-adhesive properties of the vascular endothelium yields improved renal function early after transplantation. Nature Publishing Group UK 2018-03-26 /pmc/articles/PMC5979943/ /pubmed/29581529 http://dx.doi.org/10.1038/s41598-018-21463-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nordling, Sofia Brännström, Johan Carlsson, Fredrik Lu, Bo Salvaris, Evelyn Wanders, Alkwin Buijs, Jos Estrada, Sergio Tolmachev, Vladimir Cowan, Peter J. Lorant, Tomas Magnusson, Peetra U. Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title | Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title_full | Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title_fullStr | Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title_full_unstemmed | Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title_short | Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse |
title_sort | enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: preclinical investigations in pig and mouse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979943/ https://www.ncbi.nlm.nih.gov/pubmed/29581529 http://dx.doi.org/10.1038/s41598-018-21463-1 |
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