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Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood
Granulocyte colony‐stimulating factor (G‐CSF) has been widely used in the field of allogeneic haematopoietic stem cell transplantation (allo‐HSCT) for priming donor stem cells from the bone marrow (BM) to peripheral blood (PB) to collect stem cells more conveniently. Donor‐derived natural killer (NK...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980170/ https://www.ncbi.nlm.nih.gov/pubmed/29575692 http://dx.doi.org/10.1111/jcmm.13539 |
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author | Yu, Xing‐Xing Han, Ting‐Ting Xu, Ling‐Ling Chang, Ying‐Jun Huang, Xiao‐Jun Zhao, Xiang‐Yu |
author_facet | Yu, Xing‐Xing Han, Ting‐Ting Xu, Ling‐Ling Chang, Ying‐Jun Huang, Xiao‐Jun Zhao, Xiang‐Yu |
author_sort | Yu, Xing‐Xing |
collection | PubMed |
description | Granulocyte colony‐stimulating factor (G‐CSF) has been widely used in the field of allogeneic haematopoietic stem cell transplantation (allo‐HSCT) for priming donor stem cells from the bone marrow (BM) to peripheral blood (PB) to collect stem cells more conveniently. Donor‐derived natural killer (NK) cells have important antitumour functions and immune regulatory roles post‐allo‐HSCT. The aim of this study was to evaluate the effect of G‐CSF on donors' NK cells in BM and PB. The percentage of NK cells among nuclear cells and lymphocyte was significantly decreased and led to increased ratio of T and NK cells in BM and PB post‐G‐CSF in vivo application. Relative expansion of CD56(bri) NK cells led to a decreased ratio of CD56(dim) and CD56(bri) NK subsets in BM and PB post‐G‐CSF in vivo application. The expression of CD62L, CD54, CD94, NKP30 and CXCR4 on NK cells was significantly increased in PB after G‐CSF treatment. G‐CSF treatment decreased the IFN‐γ‐secreting NK population (NK1) dramatically in BM and PB, but increased the IL‐13‐secreting NK (NK2), TGF‐β‐secreting NK (NK3) and IL‐10‐secreting NK (NKr) populations significantly in BM. Clinical data demonstrated that higher doses of NK1 infused into the allograft correlated with an increased incidence of chronic graft‐vs‐host disease post‐transplantation. Taken together, our results show that the in vivo application of G‐CSF can modulate NK subpopulations, leading to an increased ratio of T and NK cells and decreased ratio of CD56(dim) and CD56(bri) NK cells as well as decreased NK1 populations in both PB and BM. |
format | Online Article Text |
id | pubmed-5980170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59801702018-06-07 Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood Yu, Xing‐Xing Han, Ting‐Ting Xu, Ling‐Ling Chang, Ying‐Jun Huang, Xiao‐Jun Zhao, Xiang‐Yu J Cell Mol Med Original Articles Granulocyte colony‐stimulating factor (G‐CSF) has been widely used in the field of allogeneic haematopoietic stem cell transplantation (allo‐HSCT) for priming donor stem cells from the bone marrow (BM) to peripheral blood (PB) to collect stem cells more conveniently. Donor‐derived natural killer (NK) cells have important antitumour functions and immune regulatory roles post‐allo‐HSCT. The aim of this study was to evaluate the effect of G‐CSF on donors' NK cells in BM and PB. The percentage of NK cells among nuclear cells and lymphocyte was significantly decreased and led to increased ratio of T and NK cells in BM and PB post‐G‐CSF in vivo application. Relative expansion of CD56(bri) NK cells led to a decreased ratio of CD56(dim) and CD56(bri) NK subsets in BM and PB post‐G‐CSF in vivo application. The expression of CD62L, CD54, CD94, NKP30 and CXCR4 on NK cells was significantly increased in PB after G‐CSF treatment. G‐CSF treatment decreased the IFN‐γ‐secreting NK population (NK1) dramatically in BM and PB, but increased the IL‐13‐secreting NK (NK2), TGF‐β‐secreting NK (NK3) and IL‐10‐secreting NK (NKr) populations significantly in BM. Clinical data demonstrated that higher doses of NK1 infused into the allograft correlated with an increased incidence of chronic graft‐vs‐host disease post‐transplantation. Taken together, our results show that the in vivo application of G‐CSF can modulate NK subpopulations, leading to an increased ratio of T and NK cells and decreased ratio of CD56(dim) and CD56(bri) NK cells as well as decreased NK1 populations in both PB and BM. John Wiley and Sons Inc. 2018-03-25 2018-06 /pmc/articles/PMC5980170/ /pubmed/29575692 http://dx.doi.org/10.1111/jcmm.13539 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Yu, Xing‐Xing Han, Ting‐Ting Xu, Ling‐Ling Chang, Ying‐Jun Huang, Xiao‐Jun Zhao, Xiang‐Yu Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title | Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title_full | Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title_fullStr | Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title_full_unstemmed | Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title_short | Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood |
title_sort | effect of the in vivo application of granulocyte colony‐stimulating factor on nk cells in bone marrow and peripheral blood |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980170/ https://www.ncbi.nlm.nih.gov/pubmed/29575692 http://dx.doi.org/10.1111/jcmm.13539 |
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