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Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration

Spermidine has therapeutic effects in many diseases including as heart diastolic function, myopathic defects and neurodegenerative disorders via autophagy activation. Autophagy has been found to mitigate cell apoptosis in intervertebral disc degeneration (IDD). Accordingly, we theorize that spermidi...

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Autores principales: Zheng, Zengming, Wang, Zhou‐Guang, Chen, Yu, Chen, Jian, Khor, Sinan, Li, Jiawei, He, Zili, Wang, Qingqing, Zhang, Hongyu, Xu, Ke, Fanghua, Gong, Xiao, Jian, Wang, Xiangyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980193/
https://www.ncbi.nlm.nih.gov/pubmed/29575654
http://dx.doi.org/10.1111/jcmm.13586
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author Zheng, Zengming
Wang, Zhou‐Guang
Chen, Yu
Chen, Jian
Khor, Sinan
Li, Jiawei
He, Zili
Wang, Qingqing
Zhang, Hongyu
Xu, Ke
Fanghua, Gong
Xiao, Jian
Wang, Xiangyang
author_facet Zheng, Zengming
Wang, Zhou‐Guang
Chen, Yu
Chen, Jian
Khor, Sinan
Li, Jiawei
He, Zili
Wang, Qingqing
Zhang, Hongyu
Xu, Ke
Fanghua, Gong
Xiao, Jian
Wang, Xiangyang
author_sort Zheng, Zengming
collection PubMed
description Spermidine has therapeutic effects in many diseases including as heart diastolic function, myopathic defects and neurodegenerative disorders via autophagy activation. Autophagy has been found to mitigate cell apoptosis in intervertebral disc degeneration (IDD). Accordingly, we theorize that spermidine may have beneficial effects on IDD via autophagy stimulation. In this study, spermidine's effect on IDD was evaluated in tert‐butyl hydroperoxide (TBHP)‐treated nucleus pulposus cells of SD rats in vitro as well as in a puncture‐induced rat IDD model. We found that autophagy was actuated by spermidine in nucleus pulposus cells. In addition, spermidine treatment weakened the apoptotic effects of TBHP in nucleus pulposus cells. Spermidine increased the expression of anabolic proteins including Collagen‐II and aggrecan and decreased the expression of catabolic proteins including MMP13 and Adamts‐5. Additionally, autophagy blockade using 3‐MA reversed the beneficial impact of spermidine against nucleus pulposus cell apoptosis. Autophagy was thus important for spermidine's therapeutic effect on IDD. Spermidine‐treated rats had an accentuated T2‐weighted signal and a diminished histological degenerative grade than vehicle‐treated rats, showing that spermidine inhibited intervertebral disc degeneration in vivo. Thus, spermidine protects nucleus pulposus cells against apoptosis through autophagy activation and improves disc, which may be beneficial for the treatment of IDD.
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spelling pubmed-59801932018-06-07 Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration Zheng, Zengming Wang, Zhou‐Guang Chen, Yu Chen, Jian Khor, Sinan Li, Jiawei He, Zili Wang, Qingqing Zhang, Hongyu Xu, Ke Fanghua, Gong Xiao, Jian Wang, Xiangyang J Cell Mol Med Original Articles Spermidine has therapeutic effects in many diseases including as heart diastolic function, myopathic defects and neurodegenerative disorders via autophagy activation. Autophagy has been found to mitigate cell apoptosis in intervertebral disc degeneration (IDD). Accordingly, we theorize that spermidine may have beneficial effects on IDD via autophagy stimulation. In this study, spermidine's effect on IDD was evaluated in tert‐butyl hydroperoxide (TBHP)‐treated nucleus pulposus cells of SD rats in vitro as well as in a puncture‐induced rat IDD model. We found that autophagy was actuated by spermidine in nucleus pulposus cells. In addition, spermidine treatment weakened the apoptotic effects of TBHP in nucleus pulposus cells. Spermidine increased the expression of anabolic proteins including Collagen‐II and aggrecan and decreased the expression of catabolic proteins including MMP13 and Adamts‐5. Additionally, autophagy blockade using 3‐MA reversed the beneficial impact of spermidine against nucleus pulposus cell apoptosis. Autophagy was thus important for spermidine's therapeutic effect on IDD. Spermidine‐treated rats had an accentuated T2‐weighted signal and a diminished histological degenerative grade than vehicle‐treated rats, showing that spermidine inhibited intervertebral disc degeneration in vivo. Thus, spermidine protects nucleus pulposus cells against apoptosis through autophagy activation and improves disc, which may be beneficial for the treatment of IDD. John Wiley and Sons Inc. 2018-03-25 2018-06 /pmc/articles/PMC5980193/ /pubmed/29575654 http://dx.doi.org/10.1111/jcmm.13586 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zheng, Zengming
Wang, Zhou‐Guang
Chen, Yu
Chen, Jian
Khor, Sinan
Li, Jiawei
He, Zili
Wang, Qingqing
Zhang, Hongyu
Xu, Ke
Fanghua, Gong
Xiao, Jian
Wang, Xiangyang
Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title_full Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title_fullStr Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title_full_unstemmed Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title_short Spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
title_sort spermidine promotes nucleus pulposus autophagy as a protective mechanism against apoptosis and ameliorates disc degeneration
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980193/
https://www.ncbi.nlm.nih.gov/pubmed/29575654
http://dx.doi.org/10.1111/jcmm.13586
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