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A review of mycoplasma diagnostics in cattle

Mycoplasma species have a global distribution causing serious diseases in cattle worldwide including mastitis, arthritis, pneumonia, otitis media and reproductive disorders. Mycoplasma species are typically highly contagious, are capable of causing severe disease, and are difficult infections to res...

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Autores principales: Parker, Alysia M., Sheehy, Paul A., Hazelton, Mark S., Bosward, Katrina L., House, John K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980305/
https://www.ncbi.nlm.nih.gov/pubmed/29671903
http://dx.doi.org/10.1111/jvim.15135
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author Parker, Alysia M.
Sheehy, Paul A.
Hazelton, Mark S.
Bosward, Katrina L.
House, John K.
author_facet Parker, Alysia M.
Sheehy, Paul A.
Hazelton, Mark S.
Bosward, Katrina L.
House, John K.
author_sort Parker, Alysia M.
collection PubMed
description Mycoplasma species have a global distribution causing serious diseases in cattle worldwide including mastitis, arthritis, pneumonia, otitis media and reproductive disorders. Mycoplasma species are typically highly contagious, are capable of causing severe disease, and are difficult infections to resolve requiring rapid and accurate diagnosis to prevent and control disease outbreaks. This review discusses the development and use of different diagnostic methods to identify Mycoplasma species relevant to cattle, with a particular focus on Mycoplasma bovis. Traditionally, the identification and diagnosis of mycoplasma has been performed via microbial culture. More recently, the use of polymerase chain reaction to detect Mycoplasma species from various bovine samples has increased. Polymerase chain reaction has a higher efficiency, specificity, and sensitivity for laboratory diagnosis when compared with conventional culture‐based methods. Several tools are now available for typing Mycoplasma spp. isolates, allowing for genetic characterization in disease outbreak investigations. Serological diagnosis through the use of indirect ELISA allows the detection of antimycoplasma antibodies in sera and milk, with their use demonstrated on individual animal samples as well as BTM samples. While each testing method has strengths and limitations, their combined use provides complementary information, which when interpreted in conjunction with clinical signs and herd history, facilitates pathogen detection, and characterization of the disease status of cattle populations.
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spelling pubmed-59803052018-06-06 A review of mycoplasma diagnostics in cattle Parker, Alysia M. Sheehy, Paul A. Hazelton, Mark S. Bosward, Katrina L. House, John K. J Vet Intern Med FOOD AND FIBER ANIMAL Mycoplasma species have a global distribution causing serious diseases in cattle worldwide including mastitis, arthritis, pneumonia, otitis media and reproductive disorders. Mycoplasma species are typically highly contagious, are capable of causing severe disease, and are difficult infections to resolve requiring rapid and accurate diagnosis to prevent and control disease outbreaks. This review discusses the development and use of different diagnostic methods to identify Mycoplasma species relevant to cattle, with a particular focus on Mycoplasma bovis. Traditionally, the identification and diagnosis of mycoplasma has been performed via microbial culture. More recently, the use of polymerase chain reaction to detect Mycoplasma species from various bovine samples has increased. Polymerase chain reaction has a higher efficiency, specificity, and sensitivity for laboratory diagnosis when compared with conventional culture‐based methods. Several tools are now available for typing Mycoplasma spp. isolates, allowing for genetic characterization in disease outbreak investigations. Serological diagnosis through the use of indirect ELISA allows the detection of antimycoplasma antibodies in sera and milk, with their use demonstrated on individual animal samples as well as BTM samples. While each testing method has strengths and limitations, their combined use provides complementary information, which when interpreted in conjunction with clinical signs and herd history, facilitates pathogen detection, and characterization of the disease status of cattle populations. John Wiley and Sons Inc. 2018-04-19 2018 /pmc/articles/PMC5980305/ /pubmed/29671903 http://dx.doi.org/10.1111/jvim.15135 Text en Copyright © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle FOOD AND FIBER ANIMAL
Parker, Alysia M.
Sheehy, Paul A.
Hazelton, Mark S.
Bosward, Katrina L.
House, John K.
A review of mycoplasma diagnostics in cattle
title A review of mycoplasma diagnostics in cattle
title_full A review of mycoplasma diagnostics in cattle
title_fullStr A review of mycoplasma diagnostics in cattle
title_full_unstemmed A review of mycoplasma diagnostics in cattle
title_short A review of mycoplasma diagnostics in cattle
title_sort review of mycoplasma diagnostics in cattle
topic FOOD AND FIBER ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980305/
https://www.ncbi.nlm.nih.gov/pubmed/29671903
http://dx.doi.org/10.1111/jvim.15135
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