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Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy

Daphnia on the Tibetan Plateau has been little studied, and information on species diversity and biogeography is lacking. Here, we conducted a 4‐year survey using the barcoding fragment of the mitochondrial COI gene to determine the distribution and diversity of Daphnia species found across the Plat...

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Autores principales: Xu, Lei, Lin, Qiuqi, Xu, Shaolin, Gu, Yangliang, Hou, Juzhi, Liu, Yongqin, Dumont, Henri J., Han, Bo‐Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980554/
https://www.ncbi.nlm.nih.gov/pubmed/29876082
http://dx.doi.org/10.1002/ece3.4071
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author Xu, Lei
Lin, Qiuqi
Xu, Shaolin
Gu, Yangliang
Hou, Juzhi
Liu, Yongqin
Dumont, Henri J.
Han, Bo‐Ping
author_facet Xu, Lei
Lin, Qiuqi
Xu, Shaolin
Gu, Yangliang
Hou, Juzhi
Liu, Yongqin
Dumont, Henri J.
Han, Bo‐Ping
author_sort Xu, Lei
collection PubMed
description Daphnia on the Tibetan Plateau has been little studied, and information on species diversity and biogeography is lacking. Here, we conducted a 4‐year survey using the barcoding fragment of the mitochondrial COI gene to determine the distribution and diversity of Daphnia species found across the Plateau. Our results show that species richness is higher than previously thought, with total described and provisional species number doubling from 5 to 10. Six of the taxonomic units recovered by DNA taxonomy agreed well with morphology, but DNA barcoding distinguished three clades each for the D. longispina (D. galeata, D. dentifera, and D. longispina) and D. pulex (D. pulex, D. cf. tenebrosa, and D. pulicaria) complexes. The sequence divergence between congeneric species varied within a large range, from 9.25% to 30.71%. The endemic D. tibetana was the most common and widespread species, occurring in 12 hyposaline to mesosaline lakes. The lineage of D. longispina is the first confirmed occurrence in west Tibet.
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spelling pubmed-59805542018-06-06 Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy Xu, Lei Lin, Qiuqi Xu, Shaolin Gu, Yangliang Hou, Juzhi Liu, Yongqin Dumont, Henri J. Han, Bo‐Ping Ecol Evol Original Research Daphnia on the Tibetan Plateau has been little studied, and information on species diversity and biogeography is lacking. Here, we conducted a 4‐year survey using the barcoding fragment of the mitochondrial COI gene to determine the distribution and diversity of Daphnia species found across the Plateau. Our results show that species richness is higher than previously thought, with total described and provisional species number doubling from 5 to 10. Six of the taxonomic units recovered by DNA taxonomy agreed well with morphology, but DNA barcoding distinguished three clades each for the D. longispina (D. galeata, D. dentifera, and D. longispina) and D. pulex (D. pulex, D. cf. tenebrosa, and D. pulicaria) complexes. The sequence divergence between congeneric species varied within a large range, from 9.25% to 30.71%. The endemic D. tibetana was the most common and widespread species, occurring in 12 hyposaline to mesosaline lakes. The lineage of D. longispina is the first confirmed occurrence in west Tibet. John Wiley and Sons Inc. 2018-04-24 /pmc/articles/PMC5980554/ /pubmed/29876082 http://dx.doi.org/10.1002/ece3.4071 Text en © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Xu, Lei
Lin, Qiuqi
Xu, Shaolin
Gu, Yangliang
Hou, Juzhi
Liu, Yongqin
Dumont, Henri J.
Han, Bo‐Ping
Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title_full Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title_fullStr Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title_full_unstemmed Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title_short Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy
title_sort daphnia diversity on the tibetan plateau measured by dna taxonomy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980554/
https://www.ncbi.nlm.nih.gov/pubmed/29876082
http://dx.doi.org/10.1002/ece3.4071
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