Cargando…

Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi

Despite of the integrity of their RNA, some desert truffles present a non-canonical profile of rRNA where 3.3 kb is absent, 1.8 kb is clear and a band of 1.6 kb is observed. A similar rRNA profile was identified in organisms belonging to different life kingdoms, with the exception of the Kingdom Fun...

Descripción completa

Detalles Bibliográficos
Autores principales: Navarro-Ródenas, Alfonso, Carra, Andrea, Morte, Asunción
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981135/
https://www.ncbi.nlm.nih.gov/pubmed/29887836
http://dx.doi.org/10.3389/fmicb.2018.00994
_version_ 1783327982763376640
author Navarro-Ródenas, Alfonso
Carra, Andrea
Morte, Asunción
author_facet Navarro-Ródenas, Alfonso
Carra, Andrea
Morte, Asunción
author_sort Navarro-Ródenas, Alfonso
collection PubMed
description Despite of the integrity of their RNA, some desert truffles present a non-canonical profile of rRNA where 3.3 kb is absent, 1.8 kb is clear and a band of 1.6 kb is observed. A similar rRNA profile was identified in organisms belonging to different life kingdoms, with the exception of the Kingdom Fungi, as a result of a split LSU rRNA called hidden gap. rRNA profiles of desert truffles were analyzed to verify the presence of the non-canonical profile. The RNA of desert truffles and yeast were blotted and hybridized with probes complementary to LSU extremes. RACE of LSU rRNA was carried out to determine the LSU rRNA breakage point. LSU rRNA of desert truffles presents a post-transcriptional cleavage of five nucleotides that generates a hidden gap located in domain D7. LSU splits into two molecules of 1.6 and 1.8 kb. Similar to other organisms, a UAAU tract, downstream of the breakage point, was identified. Phylogenetic comparison suggests that during fungi evolution mutations were introduced in the hypervariable D7 domain, resulting in a sequence that is specifically post-transcriptionally cleaved in some desert truffles.
format Online
Article
Text
id pubmed-5981135
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-59811352018-06-08 Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi Navarro-Ródenas, Alfonso Carra, Andrea Morte, Asunción Front Microbiol Microbiology Despite of the integrity of their RNA, some desert truffles present a non-canonical profile of rRNA where 3.3 kb is absent, 1.8 kb is clear and a band of 1.6 kb is observed. A similar rRNA profile was identified in organisms belonging to different life kingdoms, with the exception of the Kingdom Fungi, as a result of a split LSU rRNA called hidden gap. rRNA profiles of desert truffles were analyzed to verify the presence of the non-canonical profile. The RNA of desert truffles and yeast were blotted and hybridized with probes complementary to LSU extremes. RACE of LSU rRNA was carried out to determine the LSU rRNA breakage point. LSU rRNA of desert truffles presents a post-transcriptional cleavage of five nucleotides that generates a hidden gap located in domain D7. LSU splits into two molecules of 1.6 and 1.8 kb. Similar to other organisms, a UAAU tract, downstream of the breakage point, was identified. Phylogenetic comparison suggests that during fungi evolution mutations were introduced in the hypervariable D7 domain, resulting in a sequence that is specifically post-transcriptionally cleaved in some desert truffles. Frontiers Media S.A. 2018-05-25 /pmc/articles/PMC5981135/ /pubmed/29887836 http://dx.doi.org/10.3389/fmicb.2018.00994 Text en Copyright © 2018 Navarro-Ródenas, Carra and Morte. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Navarro-Ródenas, Alfonso
Carra, Andrea
Morte, Asunción
Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title_full Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title_fullStr Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title_full_unstemmed Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title_short Identification of an Alternative rRNA Post-transcriptional Maturation of 26S rRNA in the Kingdom Fungi
title_sort identification of an alternative rrna post-transcriptional maturation of 26s rrna in the kingdom fungi
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981135/
https://www.ncbi.nlm.nih.gov/pubmed/29887836
http://dx.doi.org/10.3389/fmicb.2018.00994
work_keys_str_mv AT navarrorodenasalfonso identificationofanalternativerrnaposttranscriptionalmaturationof26srrnainthekingdomfungi
AT carraandrea identificationofanalternativerrnaposttranscriptionalmaturationof26srrnainthekingdomfungi
AT morteasuncion identificationofanalternativerrnaposttranscriptionalmaturationof26srrnainthekingdomfungi