Cargando…

Direct Detection of T- and B-Memory Lymphocytes by ImmunoSpot® Assays Reveals HCMV Exposure that Serum Antibodies Fail to Identify

It is essential to identify donors who have not been infected with human cytomegalovirus (HCMV) in order to avoid transmission of HCMV to recipients of blood transfusions or organ transplants. In the present study, we tested the reliability of seronegativity as an indicator for the lack of HCMV expo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Terlutter, Fredrik, Caspell, Richard, Nowacki, Tobias M., Lehmann, Alexander, Li, Ruliang, Zhang, Ting, Przybyla, Anna, Kuerten, Stefanie, Lehmann, Paul V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981269/ https://www.ncbi.nlm.nih.gov/pubmed/29783767 http://dx.doi.org/10.3390/cells7050045

Ejemplares similares

Four color B cell immunospot assays for simultaneous detection of all four immunoglobulin classes and subclasses
por: Caspell, Richard, et al.
Publicado: (2014)

Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays
por: Köppert, Sebastian, et al.
Publicado: (2021)

Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity
por: Wunsch, Marie, et al.
Publicado: (2015)

Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium
por: Waerlop, Gwenn, et al.
Publicado: (2022)

Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay
por: Wunsch, Marie, et al.
Publicado: (2015)

Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay
por: Cassaniti, Irene, et al.
Publicado: (2019)

A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool
por: Schiller, Annemarie, et al.
Publicado: (2017)

Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays
por: Calarota, Sandra A., et al.
Publicado: (2013)

Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ
por: Wang, Jann-Yuan, et al.
Publicado: (2007)

Delayed Activation Kinetics of Th2 and Th17 Cells Compared to Th1 Cells
por: Duechting, Andrea, et al.
Publicado: (2017)

High-Throughput GLP-Capable Target Cell Visualization Assay for Measuring Cell-Mediated Cytotoxicity
por: Welter, Anna, et al.
Publicado: (2018)

Interferon-γ Enzyme-Linked Immunospot Assay in Patients with Tuberculosis and Healthy Adults
por: Kim, Cheol-Hong, et al.
Publicado: (2014)

Our experience with enzyme-linked immunospot assay in the laboratory diagnostics of lyme borreliosis
por: Ondrejkova, Andrea, et al.
Publicado: (2015)

Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
por: Janetzki, Sylvia
Publicado: (2015)

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody
por: Fecher, Philipp, et al.
Publicado: (2018)

A Case of Sulfasalazine-Induced Hypersensitivity Syndrome Confirmed by Enzyme-Linked Immunospot Assay
por: Phatharacharukul, Parkpoom, et al.
Publicado: (2013)

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
por: Maggioli, Mayara F., et al.
Publicado: (2015)

Accuracy of the Bronchoalveolar Lavage Enzyme-Linked Immunospot Assay for the Diagnosis of Pulmonary Tuberculosis: A Meta-analysis
por: Pang, Caishuang, et al.
Publicado: (2016)

Value of gamma interferon enzyme-linked immunospot assay in the diagnosis of peritoneal dialysis-associated tuberculous peritonitis
por: Fan, Qiuxia, et al.
Publicado: (2021)

Development of a rapid neutralization testing system for Rhinovirus C15 based on the enzyme-linked immunospot assay
por: Zhou, Zhenhong, et al.
Publicado: (2022)

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity
por: Ozawa, Tatsuhiko, et al.
Publicado: (2012)

Utility of the Enzyme-Linked Immunospot Interferon-γ–Release Assay to Predict the Risk of Cytomegalovirus Infection in Hematopoietic Cell Transplant Recipients
por: Nesher, Lior, et al.
Publicado: (2016)

Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
por: Yuan, Kai, et al.
Publicado: (2013)

Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
por: Kuerten, Stefanie, et al.
Publicado: (2012)

IL-21 in Conjunction with Anti-CD40 and IL-4 Constitutes a Potent Polyclonal B Cell Stimulator for Monitoring Antigen-Specific Memory B Cells
por: Franke, Fridolin, et al.
Publicado: (2020)

Discordance Between the Predicted Versus the Actually Recognized CD8+ T Cell Epitopes of HCMV pp65 Antigen and Aleatory Epitope Dominance
por: Lehmann, Alexander A., et al.
Publicado: (2021)

Cultured enzyme-linked immunospot assay (ELISPOT) enhances detection of low HIV-specific immune responses in exposed seronegative individuals in Kenya
por: Muturi, Margaret, et al.
Publicado: (2006)

Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
por: Cassaniti, Irene, et al.
Publicado: (2021)

Novel microneutralization assay for HCMV using automated data collection and analysis
por: Abai, Anna Maria, et al.
Publicado: (2007)

Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients
por: Kuerten, Stefanie, et al.
Publicado: (2008)

If the antibody fails – a mass Western approach
por: Lehmann, Ute, et al.
Publicado: (2008)

ELISPOT assays with pp65 peptides or whole HCMV antigen are reliable predictors of immune control of HCMV infection in seropositive kidney transplant recipients
por: Zavaglio, Federica, et al.
Publicado: (2023)

Enumeration of Functional T-Cell Subsets by Fluorescence-Immunospot Defines Signatures of Pathogen Burden in Tuberculosis
por: Casey, Rosalyn, et al.
Publicado: (2010)

HCMV Infection and Apoptosis: How Do Monocytes Survive HCMV Infection?
por: Collins-McMillen, Donna, et al.
Publicado: (2018)

Diagnosis of tuberculous pleurisy with combination of adenosine deaminase and interferon-γ immunospot assay in a tuberculosis-endemic population: A prospective cohort study
por: Xu, Han-Yan, et al.
Publicado: (2017)

Usefulness of BK virus-specific interferon-γ enzyme-linked immunospot assay for predicting the outcome of BK virus infection in kidney transplant recipients
por: Bae, Hyunjoo, et al.
Publicado: (2021)

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells
por: Hanson, Jodi, et al.
Publicado: (2015)

Identification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes
por: Lim, Jong-Baeck, et al.
Publicado: (2009)

Comparison between enzyme‐linked immunospot assay and intracellular cytokine flow cytometry assays for the evaluation of T cell response to SARS‐CoV‐2 after symptomatic COVID‐19
por: Villemonteix, Juliette, et al.
Publicado: (2022)

Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes
por: Shibamura, Miho, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_frrvcg3cpt0m65nf8afe1dbseh