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Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)

Acyl-CoA synthetases (ACSs) are responsible for acyl-CoA synthesis from nonpolar hydrophilic fatty acids and play a vital role in many metabolic processes. As a category of ACS isozymes, members of ACS family (ACSF1-3) participate in lipid metabolism; however, their expression patterns, regulatory m...

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Autores principales: Tian, Weihua, Zheng, Hang, Yang, Liyu, Li, Hong, Tian, Yadong, Wang, Yanbin, Lyu, Shijie, Brockmann, Gudrun A., Kang, Xiangtao, Liu, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981300/
https://www.ncbi.nlm.nih.gov/pubmed/29855539
http://dx.doi.org/10.1038/s41598-018-26903-6
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author Tian, Weihua
Zheng, Hang
Yang, Liyu
Li, Hong
Tian, Yadong
Wang, Yanbin
Lyu, Shijie
Brockmann, Gudrun A.
Kang, Xiangtao
Liu, Xiaojun
author_facet Tian, Weihua
Zheng, Hang
Yang, Liyu
Li, Hong
Tian, Yadong
Wang, Yanbin
Lyu, Shijie
Brockmann, Gudrun A.
Kang, Xiangtao
Liu, Xiaojun
author_sort Tian, Weihua
collection PubMed
description Acyl-CoA synthetases (ACSs) are responsible for acyl-CoA synthesis from nonpolar hydrophilic fatty acids and play a vital role in many metabolic processes. As a category of ACS isozymes, members of ACS family (ACSF1-3) participate in lipid metabolism; however, their expression patterns, regulatory mechanisms and effects on egg-laying performance in chicken are poorly understood. Our in vivo and in vitro studies showed that ACSF1-3 genes were extensively expressed, and their expression levels changed dynamically in the liver among different development stages. Moreover, ACSF1 expression was upregulated and ACSF2 expression was downregulated by estrogen, but ACSF3 showed no response to estrogen treatment. The regulatory effect of estrogen on ACSF1 expression was mediated via ERα. The ACSF2 was highly expressed in the liver in peak-laying hens compared with pre-laying and late-laying hens, and also highly expressed in the liver continued egg-laying hens compared with inactive egg-laying hens. It is suggested that hepatic ACSF2 expression level might relate to egg-laying performance in chicken. In conclusion, the expression of ACSF1 was upregulated by estrogen via ERα, and the expression of ACSF2 was downregulated by estrogen and might be related to egg-laying performance in chicken.
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spelling pubmed-59813002018-06-06 Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus) Tian, Weihua Zheng, Hang Yang, Liyu Li, Hong Tian, Yadong Wang, Yanbin Lyu, Shijie Brockmann, Gudrun A. Kang, Xiangtao Liu, Xiaojun Sci Rep Article Acyl-CoA synthetases (ACSs) are responsible for acyl-CoA synthesis from nonpolar hydrophilic fatty acids and play a vital role in many metabolic processes. As a category of ACS isozymes, members of ACS family (ACSF1-3) participate in lipid metabolism; however, their expression patterns, regulatory mechanisms and effects on egg-laying performance in chicken are poorly understood. Our in vivo and in vitro studies showed that ACSF1-3 genes were extensively expressed, and their expression levels changed dynamically in the liver among different development stages. Moreover, ACSF1 expression was upregulated and ACSF2 expression was downregulated by estrogen, but ACSF3 showed no response to estrogen treatment. The regulatory effect of estrogen on ACSF1 expression was mediated via ERα. The ACSF2 was highly expressed in the liver in peak-laying hens compared with pre-laying and late-laying hens, and also highly expressed in the liver continued egg-laying hens compared with inactive egg-laying hens. It is suggested that hepatic ACSF2 expression level might relate to egg-laying performance in chicken. In conclusion, the expression of ACSF1 was upregulated by estrogen via ERα, and the expression of ACSF2 was downregulated by estrogen and might be related to egg-laying performance in chicken. Nature Publishing Group UK 2018-05-31 /pmc/articles/PMC5981300/ /pubmed/29855539 http://dx.doi.org/10.1038/s41598-018-26903-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tian, Weihua
Zheng, Hang
Yang, Liyu
Li, Hong
Tian, Yadong
Wang, Yanbin
Lyu, Shijie
Brockmann, Gudrun A.
Kang, Xiangtao
Liu, Xiaojun
Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title_full Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title_fullStr Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title_full_unstemmed Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title_short Dynamic Expression Profile, Regulatory Mechanism and Correlation with Egg-laying Performance of ACSF Gene Family in Chicken (Gallus gallus)
title_sort dynamic expression profile, regulatory mechanism and correlation with egg-laying performance of acsf gene family in chicken (gallus gallus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981300/
https://www.ncbi.nlm.nih.gov/pubmed/29855539
http://dx.doi.org/10.1038/s41598-018-26903-6
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