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Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control
Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. In our previous reports, we revealed the molecular mechanisms of host plant responses that underlie thermotherapy against HLB. In this study, we investigated the molecular mechanism underlying h...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981314/ https://www.ncbi.nlm.nih.gov/pubmed/29872535 http://dx.doi.org/10.1038/s41438-018-0038-x |
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author | Ding, Fang Allen, Victoria Luo, Weiqi Zhang, Shouan Duan, Yongping |
author_facet | Ding, Fang Allen, Victoria Luo, Weiqi Zhang, Shouan Duan, Yongping |
author_sort | Ding, Fang |
collection | PubMed |
description | Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. In our previous reports, we revealed the molecular mechanisms of host plant responses that underlie thermotherapy against HLB. In this study, we investigated the molecular mechanism underlying heat or tetracycline treatments on the HLB bacterium, ‘Candidatus Liberibacter asiaticus’ (Las) by focusing on Las prophage/phage conversion under stress conditions. By comparing the prophage FP1 and FP2 copy number to the copy number of 16S rDNA in HLB-affected plants, we found that the relative copy number of both FP1 and FP2 increased significantly, ranging from 3.4- to 6.7-fold change when Las-infected samples underwent a temperature shift from 23 to 37, 42 or 45 °C. When treated with tetracycline at 50–150 and 200–250 µg/ml, respectively, the relative copy number of both FP1 and FP2 increased by 3.4- to 6.0-fold. In addition, analyses of Las prophage structural gene and antirepressor gene copy numbers showed similar trends for all treatments. Furthermore, transmission electron microscopy provided direct evidence of lysogenic to lytic conversion upon temperature increase. These results not only provide new insight into the molecular mechanisms underlying heat or tetracycline treatment but also suggest a novel HLB control strategy by enhancing the endogenous conversion from Las prophages to phages. |
format | Online Article Text |
id | pubmed-5981314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59813142018-06-05 Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control Ding, Fang Allen, Victoria Luo, Weiqi Zhang, Shouan Duan, Yongping Hortic Res Article Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. In our previous reports, we revealed the molecular mechanisms of host plant responses that underlie thermotherapy against HLB. In this study, we investigated the molecular mechanism underlying heat or tetracycline treatments on the HLB bacterium, ‘Candidatus Liberibacter asiaticus’ (Las) by focusing on Las prophage/phage conversion under stress conditions. By comparing the prophage FP1 and FP2 copy number to the copy number of 16S rDNA in HLB-affected plants, we found that the relative copy number of both FP1 and FP2 increased significantly, ranging from 3.4- to 6.7-fold change when Las-infected samples underwent a temperature shift from 23 to 37, 42 or 45 °C. When treated with tetracycline at 50–150 and 200–250 µg/ml, respectively, the relative copy number of both FP1 and FP2 increased by 3.4- to 6.0-fold. In addition, analyses of Las prophage structural gene and antirepressor gene copy numbers showed similar trends for all treatments. Furthermore, transmission electron microscopy provided direct evidence of lysogenic to lytic conversion upon temperature increase. These results not only provide new insight into the molecular mechanisms underlying heat or tetracycline treatment but also suggest a novel HLB control strategy by enhancing the endogenous conversion from Las prophages to phages. Nature Publishing Group UK 2018-06-01 /pmc/articles/PMC5981314/ /pubmed/29872535 http://dx.doi.org/10.1038/s41438-018-0038-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ding, Fang Allen, Victoria Luo, Weiqi Zhang, Shouan Duan, Yongping Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title | Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title_full | Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title_fullStr | Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title_full_unstemmed | Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title_short | Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control |
title_sort | molecular mechanisms underlying heat or tetracycline treatments for citrus hlb control |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981314/ https://www.ncbi.nlm.nih.gov/pubmed/29872535 http://dx.doi.org/10.1038/s41438-018-0038-x |
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