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Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli
Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981455/ https://www.ncbi.nlm.nih.gov/pubmed/29855532 http://dx.doi.org/10.1038/s41598-018-26748-z |
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author | Arita-Morioka, Ken-ichi Yamanaka, Kunitoshi Mizunoe, Yoshimitsu Tanaka, Yoshihiko Ogura, Teru Sugimoto, Shinya |
author_facet | Arita-Morioka, Ken-ichi Yamanaka, Kunitoshi Mizunoe, Yoshimitsu Tanaka, Yoshihiko Ogura, Teru Sugimoto, Shinya |
author_sort | Arita-Morioka, Ken-ichi |
collection | PubMed |
description | Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC(50) = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC(50) = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG prevented curli production by suppressing the expression of curli-related proteins. Quantitative RT-PCR analysis revealed that the transcripts of csgA, csgB, and csgD were significantly reduced in the presence of EGCG. Interestingly, the cellular level of RpoS, a stationary-phase specific alternative sigma factor, was reduced in the presence of EGCG, whereas the rpoS transcript was not affected. Antibiotic-chase experiments and genetic analyses revealed that EGCG accelerated RpoS degradation by ATP-dependent protease ClpXP in combination with its adaptor RssB. Collectively, these results provide significant insights into the development of drugs to treat chronic biofilm-associated infections. |
format | Online Article Text |
id | pubmed-5981455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59814552018-06-06 Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli Arita-Morioka, Ken-ichi Yamanaka, Kunitoshi Mizunoe, Yoshimitsu Tanaka, Yoshihiko Ogura, Teru Sugimoto, Shinya Sci Rep Article Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC(50) = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC(50) = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG prevented curli production by suppressing the expression of curli-related proteins. Quantitative RT-PCR analysis revealed that the transcripts of csgA, csgB, and csgD were significantly reduced in the presence of EGCG. Interestingly, the cellular level of RpoS, a stationary-phase specific alternative sigma factor, was reduced in the presence of EGCG, whereas the rpoS transcript was not affected. Antibiotic-chase experiments and genetic analyses revealed that EGCG accelerated RpoS degradation by ATP-dependent protease ClpXP in combination with its adaptor RssB. Collectively, these results provide significant insights into the development of drugs to treat chronic biofilm-associated infections. Nature Publishing Group UK 2018-05-31 /pmc/articles/PMC5981455/ /pubmed/29855532 http://dx.doi.org/10.1038/s41598-018-26748-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Arita-Morioka, Ken-ichi Yamanaka, Kunitoshi Mizunoe, Yoshimitsu Tanaka, Yoshihiko Ogura, Teru Sugimoto, Shinya Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title | Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title_full | Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title_fullStr | Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title_full_unstemmed | Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title_short | Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli |
title_sort | inhibitory effects of myricetin derivatives on curli-dependent biofilm formation in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981455/ https://www.ncbi.nlm.nih.gov/pubmed/29855532 http://dx.doi.org/10.1038/s41598-018-26748-z |
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