Modulation of Cellular Response to Arsenic Trioxide Toxicity by Resveratrol

[Image: see text] Arsenic trioxide (As(2)O(3)) is an environmental carcinogen and a putative endocrine disruptor. Resveratrol has been shown to reverse As(2)O(3)-induced oxidative damage. In immortalized but nontransformed estrogen receptor α-negative human breast cells (MCF10A), we observed that 25 μM resveratrol ameliorated As(2)O(3)-induced cytotoxicity. As(2)O(3), in the presence or absence of 25 μM resveratrol, induced quinone reductase (NAD(P)H quinone dehydrogenase 1), via the induction of NFE2-related factor 2. As(2)O(3) caused a repression of cytochrome P450 (CYP)1B1, but the addition of 25 μM resveratrol rescued the expression of cytochrome P450 1B1 and kept it at a constant level. Therefore, 25 μM resveratrol can modulate the effects of As(2)O(3) on enzymes involved in estrogen metabolism.