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Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae
Given the widespread use and application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas gene editing system across many fields, a major focus has been the development, engineering and discovery of molecular means to precisely control and regulate the enzymatic function...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982135/ https://www.ncbi.nlm.nih.gov/pubmed/29488867 http://dx.doi.org/10.1099/mic.0.000635 |
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author | Basgall, Erianna M. Goetting, Samantha C. Goeckel, Megan E. Giersch, Rachael M. Roggenkamp, Emily Schrock, Madison N. Halloran, Megan Finnigan, Gregory C. |
author_facet | Basgall, Erianna M. Goetting, Samantha C. Goeckel, Megan E. Giersch, Rachael M. Roggenkamp, Emily Schrock, Madison N. Halloran, Megan Finnigan, Gregory C. |
author_sort | Basgall, Erianna M. |
collection | PubMed |
description | Given the widespread use and application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas gene editing system across many fields, a major focus has been the development, engineering and discovery of molecular means to precisely control and regulate the enzymatic function of the Cas9 nuclease. To date, a variety of Cas9 variants and fusion assemblies have been proposed to provide temporally inducible and spatially controlled editing functions. The discovery of a new class of ‘anti-CRISPR’ proteins, evolved from bacteriophage in response to the prokaryotic nuclease-based immune system, provides a new platform for control over genomic editing. One Cas9-based application of interest to the field of population control is that of the ‘gene drive’. Here, we demonstrate use of the AcrIIA2 and AcrIIA4 proteins to inhibit active gene drive systems in budding yeast. Furthermore, an unbiased mutational scan reveals that titration of Cas9 inhibition may be possible by modification of the anti-CRISPR primary sequence. |
format | Online Article Text |
id | pubmed-5982135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-59821352018-06-04 Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae Basgall, Erianna M. Goetting, Samantha C. Goeckel, Megan E. Giersch, Rachael M. Roggenkamp, Emily Schrock, Madison N. Halloran, Megan Finnigan, Gregory C. Microbiology (Reading) Research Article Given the widespread use and application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas gene editing system across many fields, a major focus has been the development, engineering and discovery of molecular means to precisely control and regulate the enzymatic function of the Cas9 nuclease. To date, a variety of Cas9 variants and fusion assemblies have been proposed to provide temporally inducible and spatially controlled editing functions. The discovery of a new class of ‘anti-CRISPR’ proteins, evolved from bacteriophage in response to the prokaryotic nuclease-based immune system, provides a new platform for control over genomic editing. One Cas9-based application of interest to the field of population control is that of the ‘gene drive’. Here, we demonstrate use of the AcrIIA2 and AcrIIA4 proteins to inhibit active gene drive systems in budding yeast. Furthermore, an unbiased mutational scan reveals that titration of Cas9 inhibition may be possible by modification of the anti-CRISPR primary sequence. Microbiology Society 2018-04 2018-02-28 /pmc/articles/PMC5982135/ /pubmed/29488867 http://dx.doi.org/10.1099/mic.0.000635 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Basgall, Erianna M. Goetting, Samantha C. Goeckel, Megan E. Giersch, Rachael M. Roggenkamp, Emily Schrock, Madison N. Halloran, Megan Finnigan, Gregory C. Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title | Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title_full | Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title_fullStr | Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title_full_unstemmed | Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title_short | Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae |
title_sort | gene drive inhibition by the anti-crispr proteins acriia2 and acriia4 in saccharomyces cerevisiae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982135/ https://www.ncbi.nlm.nih.gov/pubmed/29488867 http://dx.doi.org/10.1099/mic.0.000635 |
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