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SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics
We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on comb...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982148/ https://www.ncbi.nlm.nih.gov/pubmed/29783662 http://dx.doi.org/10.3390/s18051616 |
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author | Florinskaya, Anna Ershov, Pavel Mezentsev, Yuri Kaluzhskiy, Leonid Yablokov, Evgeniy Medvedev, Alexei Ivanov, Alexis |
author_facet | Florinskaya, Anna Ershov, Pavel Mezentsev, Yuri Kaluzhskiy, Leonid Yablokov, Evgeniy Medvedev, Alexei Ivanov, Alexis |
author_sort | Florinskaya, Anna |
collection | PubMed |
description | We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin. |
format | Online Article Text |
id | pubmed-5982148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59821482018-06-05 SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics Florinskaya, Anna Ershov, Pavel Mezentsev, Yuri Kaluzhskiy, Leonid Yablokov, Evgeniy Medvedev, Alexei Ivanov, Alexis Sensors (Basel) Review We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin. MDPI 2018-05-18 /pmc/articles/PMC5982148/ /pubmed/29783662 http://dx.doi.org/10.3390/s18051616 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Florinskaya, Anna Ershov, Pavel Mezentsev, Yuri Kaluzhskiy, Leonid Yablokov, Evgeniy Medvedev, Alexei Ivanov, Alexis SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title | SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title_full | SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title_fullStr | SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title_full_unstemmed | SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title_short | SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics |
title_sort | spr biosensors in direct molecular fishing: implications for protein interactomics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982148/ https://www.ncbi.nlm.nih.gov/pubmed/29783662 http://dx.doi.org/10.3390/s18051616 |
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