Cargando…

High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)

Improvement of resistance against rice bacterial blight (BB) disease is an important breeding strategy in breeding programs across the world, especially in Africa and southern Asia where BB is more prevalent. This report describes a high-resolution map and characterization of xa42 at XA42 locus, a r...

Descripción completa

Detalles Bibliográficos
Autores principales: Busungu, Constantine, Taura, Satoru, Sakagami, Jun-Ichi, Anai, Toyoaki, Ichitani, Katsuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982184/
https://www.ncbi.nlm.nih.gov/pubmed/29875602
http://dx.doi.org/10.1270/jsbbs.17094
_version_ 1783328187516715008
author Busungu, Constantine
Taura, Satoru
Sakagami, Jun-Ichi
Anai, Toyoaki
Ichitani, Katsuyuki
author_facet Busungu, Constantine
Taura, Satoru
Sakagami, Jun-Ichi
Anai, Toyoaki
Ichitani, Katsuyuki
author_sort Busungu, Constantine
collection PubMed
description Improvement of resistance against rice bacterial blight (BB) disease is an important breeding strategy in breeding programs across the world, especially in Africa and southern Asia where BB is more prevalent. This report describes a high-resolution map and characterization of xa42 at XA42 locus, a rice BB resistance gene in XM14, a mutant line originating from IR24. The candidate gene region was narrowed down from 582 kb, which had been obtained in our previous study, to 57 kb. XM14 shows brown spots in its leaves like lesion mimic mutants. This line also shows a shorter stature than the original cultivar IR24. In XA42 gene segregating populations, homozygotes of xa42 allele were consistently resistant to the six Japanese Xanthomonas oryzae pv. oryzae races used for this study. They also showed brown spots and markedly short stature compared with the other genotypes, suggesting that xa42 gene exhibits pleiotropic effects.
format Online
Article
Text
id pubmed-5982184
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Japanese Society of Breeding
record_format MEDLINE/PubMed
spelling pubmed-59821842018-06-06 High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.) Busungu, Constantine Taura, Satoru Sakagami, Jun-Ichi Anai, Toyoaki Ichitani, Katsuyuki Breed Sci Research Paper Improvement of resistance against rice bacterial blight (BB) disease is an important breeding strategy in breeding programs across the world, especially in Africa and southern Asia where BB is more prevalent. This report describes a high-resolution map and characterization of xa42 at XA42 locus, a rice BB resistance gene in XM14, a mutant line originating from IR24. The candidate gene region was narrowed down from 582 kb, which had been obtained in our previous study, to 57 kb. XM14 shows brown spots in its leaves like lesion mimic mutants. This line also shows a shorter stature than the original cultivar IR24. In XA42 gene segregating populations, homozygotes of xa42 allele were consistently resistant to the six Japanese Xanthomonas oryzae pv. oryzae races used for this study. They also showed brown spots and markedly short stature compared with the other genotypes, suggesting that xa42 gene exhibits pleiotropic effects. Japanese Society of Breeding 2018-03 2018-04-10 /pmc/articles/PMC5982184/ /pubmed/29875602 http://dx.doi.org/10.1270/jsbbs.17094 Text en Copyright © 2018 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Busungu, Constantine
Taura, Satoru
Sakagami, Jun-Ichi
Anai, Toyoaki
Ichitani, Katsuyuki
High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title_full High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title_fullStr High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title_full_unstemmed High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title_short High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)
title_sort high-resolution mapping and characterization of xa42, a resistance gene against multiple xanthomonas oryzae pv. oryzae races in rice (oryza sativa l.)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982184/
https://www.ncbi.nlm.nih.gov/pubmed/29875602
http://dx.doi.org/10.1270/jsbbs.17094
work_keys_str_mv AT busunguconstantine highresolutionmappingandcharacterizationofxa42aresistancegeneagainstmultiplexanthomonasoryzaepvoryzaeracesinriceoryzasatival
AT taurasatoru highresolutionmappingandcharacterizationofxa42aresistancegeneagainstmultiplexanthomonasoryzaepvoryzaeracesinriceoryzasatival
AT sakagamijunichi highresolutionmappingandcharacterizationofxa42aresistancegeneagainstmultiplexanthomonasoryzaepvoryzaeracesinriceoryzasatival
AT anaitoyoaki highresolutionmappingandcharacterizationofxa42aresistancegeneagainstmultiplexanthomonasoryzaepvoryzaeracesinriceoryzasatival
AT ichitanikatsuyuki highresolutionmappingandcharacterizationofxa42aresistancegeneagainstmultiplexanthomonasoryzaepvoryzaeracesinriceoryzasatival