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Transcriptome analysis of Pinus halepensis under drought stress and during recovery

Forest trees use various strategies to cope with drought stress and these strategies involve complex molecular mechanisms. Pinus halepensis Miller (Aleppo pine) is found throughout the Mediterranean basin and is one of the most drought-tolerant pine species. In order to decipher the molecular mechan...

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Autores principales: Fox, Hagar, Doron-Faigenboim, Adi, Kelly, Gilor, Bourstein, Ronny, Attia, Ziv, Zhou, Jing, Moshe, Yosef, Moshelion, Menachem, David-Schwartz, Rakefet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982726/
https://www.ncbi.nlm.nih.gov/pubmed/29177514
http://dx.doi.org/10.1093/treephys/tpx137
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author Fox, Hagar
Doron-Faigenboim, Adi
Kelly, Gilor
Bourstein, Ronny
Attia, Ziv
Zhou, Jing
Moshe, Yosef
Moshelion, Menachem
David-Schwartz, Rakefet
author_facet Fox, Hagar
Doron-Faigenboim, Adi
Kelly, Gilor
Bourstein, Ronny
Attia, Ziv
Zhou, Jing
Moshe, Yosef
Moshelion, Menachem
David-Schwartz, Rakefet
author_sort Fox, Hagar
collection PubMed
description Forest trees use various strategies to cope with drought stress and these strategies involve complex molecular mechanisms. Pinus halepensis Miller (Aleppo pine) is found throughout the Mediterranean basin and is one of the most drought-tolerant pine species. In order to decipher the molecular mechanisms that P. halepensis uses to withstand drought, we performed large-scale physiological and transcriptome analyses. We selected a mature tree from a semi-arid area with suboptimal growth conditions for clonal propagation through cuttings. We then used a high-throughput experimental system to continuously monitor whole-plant transpiration rates, stomatal conductance and the vapor pressure deficit. The transcriptomes of plants were examined at six physiological stages: pre-stomatal response, partial stomatal closure, minimum transpiration, post-irrigation, partial recovery and full recovery. At each stage, data from plants exposed to the drought treatment were compared with data collected from well-irrigated control plants. A drought-stressed P. halepensis transcriptome was created using paired-end RNA-seq. In total, ~6000 differentially expressed, non-redundant transcripts were identified between drought-treated and control trees. Cluster analysis has revealed stress-induced down-regulation of transcripts related to photosynthesis, reactive oxygen species (ROS)-scavenging through the ascorbic acid (AsA)-glutathione cycle, fatty acid and cell wall biosynthesis, stomatal activity, and the biosynthesis of flavonoids and terpenoids. Up-regulated processes included chlorophyll degradation, ROS-scavenging through AsA-independent thiol-mediated pathways, abscisic acid response and accumulation of heat shock proteins, thaumatin and exordium. Recovery from drought induced strong transcription of retrotransposons, especially the retrovirus-related transposon Tnt1-94. The drought-related transcriptome illustrates this species’ dynamic response to drought and recovery and unravels novel mechanisms.
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spelling pubmed-59827262018-06-06 Transcriptome analysis of Pinus halepensis under drought stress and during recovery Fox, Hagar Doron-Faigenboim, Adi Kelly, Gilor Bourstein, Ronny Attia, Ziv Zhou, Jing Moshe, Yosef Moshelion, Menachem David-Schwartz, Rakefet Tree Physiol Research Paper Forest trees use various strategies to cope with drought stress and these strategies involve complex molecular mechanisms. Pinus halepensis Miller (Aleppo pine) is found throughout the Mediterranean basin and is one of the most drought-tolerant pine species. In order to decipher the molecular mechanisms that P. halepensis uses to withstand drought, we performed large-scale physiological and transcriptome analyses. We selected a mature tree from a semi-arid area with suboptimal growth conditions for clonal propagation through cuttings. We then used a high-throughput experimental system to continuously monitor whole-plant transpiration rates, stomatal conductance and the vapor pressure deficit. The transcriptomes of plants were examined at six physiological stages: pre-stomatal response, partial stomatal closure, minimum transpiration, post-irrigation, partial recovery and full recovery. At each stage, data from plants exposed to the drought treatment were compared with data collected from well-irrigated control plants. A drought-stressed P. halepensis transcriptome was created using paired-end RNA-seq. In total, ~6000 differentially expressed, non-redundant transcripts were identified between drought-treated and control trees. Cluster analysis has revealed stress-induced down-regulation of transcripts related to photosynthesis, reactive oxygen species (ROS)-scavenging through the ascorbic acid (AsA)-glutathione cycle, fatty acid and cell wall biosynthesis, stomatal activity, and the biosynthesis of flavonoids and terpenoids. Up-regulated processes included chlorophyll degradation, ROS-scavenging through AsA-independent thiol-mediated pathways, abscisic acid response and accumulation of heat shock proteins, thaumatin and exordium. Recovery from drought induced strong transcription of retrotransposons, especially the retrovirus-related transposon Tnt1-94. The drought-related transcriptome illustrates this species’ dynamic response to drought and recovery and unravels novel mechanisms. Oxford University Press 2017-11-21 /pmc/articles/PMC5982726/ /pubmed/29177514 http://dx.doi.org/10.1093/treephys/tpx137 Text en © The Author 2017. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Paper
Fox, Hagar
Doron-Faigenboim, Adi
Kelly, Gilor
Bourstein, Ronny
Attia, Ziv
Zhou, Jing
Moshe, Yosef
Moshelion, Menachem
David-Schwartz, Rakefet
Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title_full Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title_fullStr Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title_full_unstemmed Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title_short Transcriptome analysis of Pinus halepensis under drought stress and during recovery
title_sort transcriptome analysis of pinus halepensis under drought stress and during recovery
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982726/
https://www.ncbi.nlm.nih.gov/pubmed/29177514
http://dx.doi.org/10.1093/treephys/tpx137
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