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Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation

To examine the relationship between local oxygen partial pressure and energy metabolism in the tumor, electron paramagnetic resonance imaging (EPRI) and magnetic resonance imaging (MRI) with hyperpolarized [1-(13)C] pyruvate were performed. SCCVII and HT29 solid tumors implanted in the mouse leg wer...

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Autores principales: Matsuo, Masayuki, Kawai, Tatsuya, Kishimoto, Shun, Saito, Keita, Munasinghe, Jeeva, Devasahayam, Nallathamby, Mitchell, James B., Krishna, Murali C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982751/
https://www.ncbi.nlm.nih.gov/pubmed/29861855
http://dx.doi.org/10.18632/oncotarget.25317
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author Matsuo, Masayuki
Kawai, Tatsuya
Kishimoto, Shun
Saito, Keita
Munasinghe, Jeeva
Devasahayam, Nallathamby
Mitchell, James B.
Krishna, Murali C.
author_facet Matsuo, Masayuki
Kawai, Tatsuya
Kishimoto, Shun
Saito, Keita
Munasinghe, Jeeva
Devasahayam, Nallathamby
Mitchell, James B.
Krishna, Murali C.
author_sort Matsuo, Masayuki
collection PubMed
description To examine the relationship between local oxygen partial pressure and energy metabolism in the tumor, electron paramagnetic resonance imaging (EPRI) and magnetic resonance imaging (MRI) with hyperpolarized [1-(13)C] pyruvate were performed. SCCVII and HT29 solid tumors implanted in the mouse leg were imaged by EPRI using OX063, a paramagnetic probe and (13)C-MRI using hyperpolarized [1-(13)C] pyruvate. Local partial oxygen pressure and pyruvate metabolism in the two tumor implants were examined. The effect of a single dose of 5-Gy irradiation on the pO(2) and metabolism was also investigated by sequential imaging of EPRI and (13)C-MRI in HT29 tumors. A phantom study using tubes filled with different concentration of [1-(13)C] pyruvate, [1-(13)C] lactate, and OX063 at different levels of oxygen confirmed the validity of this sequential imaging of EPRI and hyperpolarized (13)C-MRI. In vivo studies revealed SCCVII tumor had a significantly larger hypoxic fraction (pO(2) < 8 mmHg) compared to HT29 tumor. The flux of pyruvate-to-lactate conversion was also higher in SCCVII than HT29. The lactate-to-pyruvate ratio in hypoxic regions (pO(2) < 8 mmHg) 24 hours after 5-Gy irradiation was significantly higher than those without irradiation (0.76 vs. 0.36) in HT29 tumor. The in vitro study showed an increase in extracellular acidification rate after irradiation. In conclusion, co-imaging of pO(2) and pyruvate-to-lactate conversion kinetics successfully showed the local metabolic changes especially in hypoxic area induced by radiation therapy.
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spelling pubmed-59827512018-06-01 Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation Matsuo, Masayuki Kawai, Tatsuya Kishimoto, Shun Saito, Keita Munasinghe, Jeeva Devasahayam, Nallathamby Mitchell, James B. Krishna, Murali C. Oncotarget Research Paper To examine the relationship between local oxygen partial pressure and energy metabolism in the tumor, electron paramagnetic resonance imaging (EPRI) and magnetic resonance imaging (MRI) with hyperpolarized [1-(13)C] pyruvate were performed. SCCVII and HT29 solid tumors implanted in the mouse leg were imaged by EPRI using OX063, a paramagnetic probe and (13)C-MRI using hyperpolarized [1-(13)C] pyruvate. Local partial oxygen pressure and pyruvate metabolism in the two tumor implants were examined. The effect of a single dose of 5-Gy irradiation on the pO(2) and metabolism was also investigated by sequential imaging of EPRI and (13)C-MRI in HT29 tumors. A phantom study using tubes filled with different concentration of [1-(13)C] pyruvate, [1-(13)C] lactate, and OX063 at different levels of oxygen confirmed the validity of this sequential imaging of EPRI and hyperpolarized (13)C-MRI. In vivo studies revealed SCCVII tumor had a significantly larger hypoxic fraction (pO(2) < 8 mmHg) compared to HT29 tumor. The flux of pyruvate-to-lactate conversion was also higher in SCCVII than HT29. The lactate-to-pyruvate ratio in hypoxic regions (pO(2) < 8 mmHg) 24 hours after 5-Gy irradiation was significantly higher than those without irradiation (0.76 vs. 0.36) in HT29 tumor. The in vitro study showed an increase in extracellular acidification rate after irradiation. In conclusion, co-imaging of pO(2) and pyruvate-to-lactate conversion kinetics successfully showed the local metabolic changes especially in hypoxic area induced by radiation therapy. Impact Journals LLC 2018-05-18 /pmc/articles/PMC5982751/ /pubmed/29861855 http://dx.doi.org/10.18632/oncotarget.25317 Text en Copyright: © 2018 Matsuo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Matsuo, Masayuki
Kawai, Tatsuya
Kishimoto, Shun
Saito, Keita
Munasinghe, Jeeva
Devasahayam, Nallathamby
Mitchell, James B.
Krishna, Murali C.
Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title_full Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title_fullStr Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title_full_unstemmed Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title_short Co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-C hyperpolarized magnetic resonance imaging before and after irradiation
title_sort co-imaging of the tumor oxygenation and metabolism using electron paramagnetic resonance imaging and 13-c hyperpolarized magnetic resonance imaging before and after irradiation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982751/
https://www.ncbi.nlm.nih.gov/pubmed/29861855
http://dx.doi.org/10.18632/oncotarget.25317
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