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Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer

Sorting nexin-1 (SNX1) is an important functional protein in cell endocytosis, efflux, protein sorting, cell signal transduction, etc; however, the expression, the role and clinical relevance of SNX1 have not been investigated in gastric cancer (GC). In this study, we first performed a bioinformatic...

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Autores principales: Zhan, Xiao-Yong, Zhang, Yaqiong, Zhai, Ertao, Zhu, Qing-Yi, He, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983015/
https://www.ncbi.nlm.nih.gov/pubmed/29868263
http://dx.doi.org/10.7717/peerj.4829
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author Zhan, Xiao-Yong
Zhang, Yaqiong
Zhai, Ertao
Zhu, Qing-Yi
He, Yulong
author_facet Zhan, Xiao-Yong
Zhang, Yaqiong
Zhai, Ertao
Zhu, Qing-Yi
He, Yulong
author_sort Zhan, Xiao-Yong
collection PubMed
description Sorting nexin-1 (SNX1) is an important functional protein in cell endocytosis, efflux, protein sorting, cell signal transduction, etc; however, the expression, the role and clinical relevance of SNX1 have not been investigated in gastric cancer (GC). In this study, we first performed a bioinformatics investigation using the data obtained from The Cancer Genome Atlas (TCGA) database. The result showed that SNX1 mRNA levels were significantly lower in GC tissues than in paracancerous tissues. In a study of 150 cases of GC, including 60 cases with paired paracancerous and cancer tissues and 90 cases with detailed follow-up information, SNX1 expression was analyzed by immunohistochemistry. Our study on paired paracancerous and cancer tissues showed that SNX1 protein expression remarkably decreased in GC tissues (50/60, 83.33%). A study on 90 patients with detailed follow-up information showed that tumors with higher SNX1 protein level were correlated with better clinicopathologic stages (p = 0.0285), nodal status (p = 0.0286), smaller tumor sizes (p = 0.0294) and a better survival rate in patients with GC (p = 0.0245). Univariate analysis of the 90 patients with GC showed that low-level SNX1 was significantly correlated with decreased overall survival of GC patients (p = 0.008), and associated with a relatively higher cumulative hazard of death. Exogenous expression of SNX1 inhibited the growth, migration, invasion and promoted the apoptosis and enhanced the sensitivity of GC cells to the chemotherapeutic drug 5-Fluorouracil (5-Fu) in vitro, while knockdown of SNX1 by short hairpin RNA (shRNA) significantly promoted the growth, migration, invasion and reduced the apoptosis and the sensitivity of GC cells to 5-Fu. SNX1 also showed to influence the levels of epithelial-mesenchymal transition markers including Vimentin, Snail, and E-cadherin in GC cells in vitro. Taken together, we propose here that SNX1 serves as a tumor suppressor and prognostic marker that reduces tumor cell malignancy for GC.
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spelling pubmed-59830152018-06-04 Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer Zhan, Xiao-Yong Zhang, Yaqiong Zhai, Ertao Zhu, Qing-Yi He, Yulong PeerJ Gastroenterology and Hepatology Sorting nexin-1 (SNX1) is an important functional protein in cell endocytosis, efflux, protein sorting, cell signal transduction, etc; however, the expression, the role and clinical relevance of SNX1 have not been investigated in gastric cancer (GC). In this study, we first performed a bioinformatics investigation using the data obtained from The Cancer Genome Atlas (TCGA) database. The result showed that SNX1 mRNA levels were significantly lower in GC tissues than in paracancerous tissues. In a study of 150 cases of GC, including 60 cases with paired paracancerous and cancer tissues and 90 cases with detailed follow-up information, SNX1 expression was analyzed by immunohistochemistry. Our study on paired paracancerous and cancer tissues showed that SNX1 protein expression remarkably decreased in GC tissues (50/60, 83.33%). A study on 90 patients with detailed follow-up information showed that tumors with higher SNX1 protein level were correlated with better clinicopathologic stages (p = 0.0285), nodal status (p = 0.0286), smaller tumor sizes (p = 0.0294) and a better survival rate in patients with GC (p = 0.0245). Univariate analysis of the 90 patients with GC showed that low-level SNX1 was significantly correlated with decreased overall survival of GC patients (p = 0.008), and associated with a relatively higher cumulative hazard of death. Exogenous expression of SNX1 inhibited the growth, migration, invasion and promoted the apoptosis and enhanced the sensitivity of GC cells to the chemotherapeutic drug 5-Fluorouracil (5-Fu) in vitro, while knockdown of SNX1 by short hairpin RNA (shRNA) significantly promoted the growth, migration, invasion and reduced the apoptosis and the sensitivity of GC cells to 5-Fu. SNX1 also showed to influence the levels of epithelial-mesenchymal transition markers including Vimentin, Snail, and E-cadherin in GC cells in vitro. Taken together, we propose here that SNX1 serves as a tumor suppressor and prognostic marker that reduces tumor cell malignancy for GC. PeerJ Inc. 2018-05-29 /pmc/articles/PMC5983015/ /pubmed/29868263 http://dx.doi.org/10.7717/peerj.4829 Text en ©2018 Zhan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Gastroenterology and Hepatology
Zhan, Xiao-Yong
Zhang, Yaqiong
Zhai, Ertao
Zhu, Qing-Yi
He, Yulong
Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title_full Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title_fullStr Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title_full_unstemmed Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title_short Sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
title_sort sorting nexin-1 is a candidate tumor suppressor and potential prognostic marker in gastric cancer
topic Gastroenterology and Hepatology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983015/
https://www.ncbi.nlm.nih.gov/pubmed/29868263
http://dx.doi.org/10.7717/peerj.4829
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