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Nanobody Technology for Mycotoxin Detection: Current Status and Prospects
Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. Howeve...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983236/ https://www.ncbi.nlm.nih.gov/pubmed/29710823 http://dx.doi.org/10.3390/toxins10050180 |
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author | He, Ting Zhu, Jiang Nie, Yao Hu, Rui Wang, Ting Li, Peiwu Zhang, Qi Yang, Yunhuang |
author_facet | He, Ting Zhu, Jiang Nie, Yao Hu, Rui Wang, Ting Li, Peiwu Zhang, Qi Yang, Yunhuang |
author_sort | He, Ting |
collection | PubMed |
description | Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. However, conventional antibodies involved in immunoassays are difficult to be expressed recombinantly and are susceptible to harsh environments. Nanobodies (or VHH antibodies) are antigen-binding sites of the heavy-chain antibodies produced from Camelidae. They are found to be expressed easily in prokaryotic or eukaryotic expression systems, more robust in extreme conditions, and facile to be used as surrogates for artificial antigens. These properties make them the promising and environmentally friendly immunoreagents in the next generation of immunoassays. This review briefly describes the latest developments in the area of nanobodies used in mycotoxin detection. Moreover, by integrating the introduction of the principle of nanobodies production and the critical assessment of their performance, this paper also proposes the prospect of nanobodies in the field of food safety in the foreseeable future. |
format | Online Article Text |
id | pubmed-5983236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59832362018-06-06 Nanobody Technology for Mycotoxin Detection: Current Status and Prospects He, Ting Zhu, Jiang Nie, Yao Hu, Rui Wang, Ting Li, Peiwu Zhang, Qi Yang, Yunhuang Toxins (Basel) Review Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. However, conventional antibodies involved in immunoassays are difficult to be expressed recombinantly and are susceptible to harsh environments. Nanobodies (or VHH antibodies) are antigen-binding sites of the heavy-chain antibodies produced from Camelidae. They are found to be expressed easily in prokaryotic or eukaryotic expression systems, more robust in extreme conditions, and facile to be used as surrogates for artificial antigens. These properties make them the promising and environmentally friendly immunoreagents in the next generation of immunoassays. This review briefly describes the latest developments in the area of nanobodies used in mycotoxin detection. Moreover, by integrating the introduction of the principle of nanobodies production and the critical assessment of their performance, this paper also proposes the prospect of nanobodies in the field of food safety in the foreseeable future. MDPI 2018-04-29 /pmc/articles/PMC5983236/ /pubmed/29710823 http://dx.doi.org/10.3390/toxins10050180 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review He, Ting Zhu, Jiang Nie, Yao Hu, Rui Wang, Ting Li, Peiwu Zhang, Qi Yang, Yunhuang Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title | Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title_full | Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title_fullStr | Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title_full_unstemmed | Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title_short | Nanobody Technology for Mycotoxin Detection: Current Status and Prospects |
title_sort | nanobody technology for mycotoxin detection: current status and prospects |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983236/ https://www.ncbi.nlm.nih.gov/pubmed/29710823 http://dx.doi.org/10.3390/toxins10050180 |
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