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Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan

Bacteria biofilm helps bacteria prevent phagocytosis during infection and increase resistance to antibiotics. Staphylococcus aureus is a Gram-positive pathogenic bacterium and is tightly associated with biofilm-related infections, which have led to great threat to human health. Chitosan, the only ca...

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Autores principales: Zhang, Guiqiang, Liu, Jing, Li, Ruilian, Jiao, Siming, Feng, Cui, Wang, Zhuo A., Du, Yuguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983282/
https://www.ncbi.nlm.nih.gov/pubmed/29734657
http://dx.doi.org/10.3390/md16050151
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author Zhang, Guiqiang
Liu, Jing
Li, Ruilian
Jiao, Siming
Feng, Cui
Wang, Zhuo A.
Du, Yuguang
author_facet Zhang, Guiqiang
Liu, Jing
Li, Ruilian
Jiao, Siming
Feng, Cui
Wang, Zhuo A.
Du, Yuguang
author_sort Zhang, Guiqiang
collection PubMed
description Bacteria biofilm helps bacteria prevent phagocytosis during infection and increase resistance to antibiotics. Staphylococcus aureus is a Gram-positive pathogenic bacterium and is tightly associated with biofilm-related infections, which have led to great threat to human health. Chitosan, the only cationic polysaccharide in nature, has been demonstrated to have antimicrobial and anti-biofilm activities, which, however, require a relative high dosage of chitosan. Moreover, poor water solubility further restricts its applications on anti-infection therapy. Inulins are a group of polysaccharides produced by many types of plants, and are widely used in processed foods. Compared to chitosan, inulin is very soluble in water and possesses a mild antibacterial activity against certain pathogenic bacteria. In order to develop an effective strategy to treat biofilm-related infections, we introduce a method by covalent conjugation of inulin to chitosan. The physicochemical characterization of the inulin–chitosan conjugate was assayed, and the anti-biofilm activity was evaluated against S. aureus biofilm. The results indicated that, as compared to chitosan, this novel polysaccharide–polysaccharide conjugate significantly enhanced activities against S. aureus either in a biofilm or planktonic state. Of note, the conjugate also showed a broad spectrum anti-biofilm activity on different bacteria strains and low cellular toxicity to mammalian cells. These results suggested that chitosan conjugation of inulin was a viable strategy for treatment against biofilm-related infections. This finding may further spread the application of natural polysaccharides on treatments of infectious disease.
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spelling pubmed-59832822018-06-06 Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan Zhang, Guiqiang Liu, Jing Li, Ruilian Jiao, Siming Feng, Cui Wang, Zhuo A. Du, Yuguang Mar Drugs Article Bacteria biofilm helps bacteria prevent phagocytosis during infection and increase resistance to antibiotics. Staphylococcus aureus is a Gram-positive pathogenic bacterium and is tightly associated with biofilm-related infections, which have led to great threat to human health. Chitosan, the only cationic polysaccharide in nature, has been demonstrated to have antimicrobial and anti-biofilm activities, which, however, require a relative high dosage of chitosan. Moreover, poor water solubility further restricts its applications on anti-infection therapy. Inulins are a group of polysaccharides produced by many types of plants, and are widely used in processed foods. Compared to chitosan, inulin is very soluble in water and possesses a mild antibacterial activity against certain pathogenic bacteria. In order to develop an effective strategy to treat biofilm-related infections, we introduce a method by covalent conjugation of inulin to chitosan. The physicochemical characterization of the inulin–chitosan conjugate was assayed, and the anti-biofilm activity was evaluated against S. aureus biofilm. The results indicated that, as compared to chitosan, this novel polysaccharide–polysaccharide conjugate significantly enhanced activities against S. aureus either in a biofilm or planktonic state. Of note, the conjugate also showed a broad spectrum anti-biofilm activity on different bacteria strains and low cellular toxicity to mammalian cells. These results suggested that chitosan conjugation of inulin was a viable strategy for treatment against biofilm-related infections. This finding may further spread the application of natural polysaccharides on treatments of infectious disease. MDPI 2018-05-04 /pmc/articles/PMC5983282/ /pubmed/29734657 http://dx.doi.org/10.3390/md16050151 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Guiqiang
Liu, Jing
Li, Ruilian
Jiao, Siming
Feng, Cui
Wang, Zhuo A.
Du, Yuguang
Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title_full Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title_fullStr Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title_full_unstemmed Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title_short Conjugation of Inulin Improves Anti-Biofilm Activity of Chitosan
title_sort conjugation of inulin improves anti-biofilm activity of chitosan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983282/
https://www.ncbi.nlm.nih.gov/pubmed/29734657
http://dx.doi.org/10.3390/md16050151
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