High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide

An alginate lyase encoding gene sagl from Flavobacterium sp. H63 was codon optimized and recombinantly expressed at high level in P.pastoris through high cell-density fermentation. The highest yield of recombinant enzyme of sagl (rSAGL) in yeast culture supernatant reached 226.4 μg/mL (915.5 U/mL)....

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Detalles Bibliográficos
Autores principales: Li, Haifeng, Wang, Shuling, Zhang, Yunyi, Chen, Liehuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983289/
https://www.ncbi.nlm.nih.gov/pubmed/29751659
http://dx.doi.org/10.3390/md16050158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983289/
https://www.ncbi.nlm.nih.gov/pubmed/29751659
http://dx.doi.org/10.3390/md16050158

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