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A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight
Fusarium head blight (FHB) mainly caused by F. graminearum, always brings serious damage to wheat production worldwide. In this study, we found that strain LM2303 had strong antagonist activity against F. graminearum and significantly reduced disease severity of FHB with the control efficiency of 72...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983450/ https://www.ncbi.nlm.nih.gov/pubmed/29856856 http://dx.doi.org/10.1371/journal.pone.0198560 |
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author | Chen, Liang Heng, Junying Qin, Suya Bian, Ke |
author_facet | Chen, Liang Heng, Junying Qin, Suya Bian, Ke |
author_sort | Chen, Liang |
collection | PubMed |
description | Fusarium head blight (FHB) mainly caused by F. graminearum, always brings serious damage to wheat production worldwide. In this study, we found that strain LM2303 had strong antagonist activity against F. graminearum and significantly reduced disease severity of FHB with the control efficiency of 72.3% under field conditions. To gain a comprehensive understanding of the biocontrol potential of strain LM2303 against FHB, an integrated approach of genome mining and chemical analysis was employed. The whole genome of strain LM2303 was obtained and analyzed, showing the largest number of genes/gene clusters associated with biocontrol functions as compared with the known biocontrol strains (FZB42, M75, CAU B946). And strain LM2303 was accurately determined as a member of the B. velezensis clade using the phylogenomic analysis of single-copy core genes. Through genome mining, 13 biosynthetic gene clusters(BGCs) encoding secondary metabolites with biocontrol functions were identified, which were further confirmed through chemical analyses such as UHPLC-ESI-MS, including three antifungal metabolites (fengycin B, iturin A, and surfactin A), eight antibacterial metabolites (surfactin A, butirosin, plantazolicin and hydrolyzed plantazolicin, kijanimicin, bacilysin, difficidin, bacillaene A and bacillaene B, 7-o-malonyl macrolactin A and 7-o-succinyl macrolactin A), the siderophore bacillibactin, molybdenum cofactor and teichuronic acid. In addition, genes/gene clusters involved in plant colonization, plant growth promotion and induced systemic resistance were also found and analyzed, along with the corresponding metabolites. Finally, four different mechanisms of strain LM2303 involved in the biocontrol of FHB were putatively obtained. This work provides better insights into a mechanistic understanding of strain LM2303 in control of FHB, reinforcing the higher potential of this strain as a powerful biocontrol strain agent (BCA) for FHB control. The results also provide scientific reference and comparison for other biocontrol strains. |
format | Online Article Text |
id | pubmed-5983450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59834502018-06-17 A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight Chen, Liang Heng, Junying Qin, Suya Bian, Ke PLoS One Research Article Fusarium head blight (FHB) mainly caused by F. graminearum, always brings serious damage to wheat production worldwide. In this study, we found that strain LM2303 had strong antagonist activity against F. graminearum and significantly reduced disease severity of FHB with the control efficiency of 72.3% under field conditions. To gain a comprehensive understanding of the biocontrol potential of strain LM2303 against FHB, an integrated approach of genome mining and chemical analysis was employed. The whole genome of strain LM2303 was obtained and analyzed, showing the largest number of genes/gene clusters associated with biocontrol functions as compared with the known biocontrol strains (FZB42, M75, CAU B946). And strain LM2303 was accurately determined as a member of the B. velezensis clade using the phylogenomic analysis of single-copy core genes. Through genome mining, 13 biosynthetic gene clusters(BGCs) encoding secondary metabolites with biocontrol functions were identified, which were further confirmed through chemical analyses such as UHPLC-ESI-MS, including three antifungal metabolites (fengycin B, iturin A, and surfactin A), eight antibacterial metabolites (surfactin A, butirosin, plantazolicin and hydrolyzed plantazolicin, kijanimicin, bacilysin, difficidin, bacillaene A and bacillaene B, 7-o-malonyl macrolactin A and 7-o-succinyl macrolactin A), the siderophore bacillibactin, molybdenum cofactor and teichuronic acid. In addition, genes/gene clusters involved in plant colonization, plant growth promotion and induced systemic resistance were also found and analyzed, along with the corresponding metabolites. Finally, four different mechanisms of strain LM2303 involved in the biocontrol of FHB were putatively obtained. This work provides better insights into a mechanistic understanding of strain LM2303 in control of FHB, reinforcing the higher potential of this strain as a powerful biocontrol strain agent (BCA) for FHB control. The results also provide scientific reference and comparison for other biocontrol strains. Public Library of Science 2018-06-01 /pmc/articles/PMC5983450/ /pubmed/29856856 http://dx.doi.org/10.1371/journal.pone.0198560 Text en © 2018 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chen, Liang Heng, Junying Qin, Suya Bian, Ke A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title | A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title_full | A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title_fullStr | A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title_full_unstemmed | A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title_short | A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight |
title_sort | comprehensive understanding of the biocontrol potential of bacillus velezensis lm2303 against fusarium head blight |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983450/ https://www.ncbi.nlm.nih.gov/pubmed/29856856 http://dx.doi.org/10.1371/journal.pone.0198560 |
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