Cargando…

Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry

Sphingomyelin- and cholesterol- enriched membrane domains, commonly referred to as “rafts” play a crucial role in a large number of intra- and intercellular processes. Recent experiments suggest that not only the volumetric inhomogeneity of lipid distribution in rafts, but also the arrangement of th...

Descripción completa

Detalles Bibliográficos
Autores principales: Molotkovsky, Rodion J., Alexandrova, Veronika V., Galimzyanov, Timur R., Jiménez-Munguía, Irene, Pavlov, Konstantin V., Batishchev, Oleg V., Akimov, Sergey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983600/
https://www.ncbi.nlm.nih.gov/pubmed/29772704
http://dx.doi.org/10.3390/ijms19051483
_version_ 1783328455058784256
author Molotkovsky, Rodion J.
Alexandrova, Veronika V.
Galimzyanov, Timur R.
Jiménez-Munguía, Irene
Pavlov, Konstantin V.
Batishchev, Oleg V.
Akimov, Sergey A.
author_facet Molotkovsky, Rodion J.
Alexandrova, Veronika V.
Galimzyanov, Timur R.
Jiménez-Munguía, Irene
Pavlov, Konstantin V.
Batishchev, Oleg V.
Akimov, Sergey A.
author_sort Molotkovsky, Rodion J.
collection PubMed
description Sphingomyelin- and cholesterol- enriched membrane domains, commonly referred to as “rafts” play a crucial role in a large number of intra- and intercellular processes. Recent experiments suggest that not only the volumetric inhomogeneity of lipid distribution in rafts, but also the arrangement of the 1D boundary between the raft and the surrounding membrane is important for the membrane-associated processes. The reason is that the boundary preferentially recruits different peptides, such as HIV (human immunodeficiency virus) fusion peptide. In the present work, we report a theoretical investigation of mechanisms of influence of the raft boundary arrangement upon virus-induced membrane fusion. We theoretically predict that the raft boundary can act as an attractor for viral fusion peptides, which preferentially distribute into the vicinity of the boundary, playing the role of ‘line active components’ of the membrane (‘linactants’). We have calculated the height of the fusion energy barrier and demonstrated that, in the case of fusion between HIV membrane and the target cell, presence of the raft boundary in the vicinity of the fusion site facilitates fusion. The results we obtained can be further generalized to be applicable to other enveloped viruses.
format Online
Article
Text
id pubmed-5983600
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59836002018-06-05 Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry Molotkovsky, Rodion J. Alexandrova, Veronika V. Galimzyanov, Timur R. Jiménez-Munguía, Irene Pavlov, Konstantin V. Batishchev, Oleg V. Akimov, Sergey A. Int J Mol Sci Article Sphingomyelin- and cholesterol- enriched membrane domains, commonly referred to as “rafts” play a crucial role in a large number of intra- and intercellular processes. Recent experiments suggest that not only the volumetric inhomogeneity of lipid distribution in rafts, but also the arrangement of the 1D boundary between the raft and the surrounding membrane is important for the membrane-associated processes. The reason is that the boundary preferentially recruits different peptides, such as HIV (human immunodeficiency virus) fusion peptide. In the present work, we report a theoretical investigation of mechanisms of influence of the raft boundary arrangement upon virus-induced membrane fusion. We theoretically predict that the raft boundary can act as an attractor for viral fusion peptides, which preferentially distribute into the vicinity of the boundary, playing the role of ‘line active components’ of the membrane (‘linactants’). We have calculated the height of the fusion energy barrier and demonstrated that, in the case of fusion between HIV membrane and the target cell, presence of the raft boundary in the vicinity of the fusion site facilitates fusion. The results we obtained can be further generalized to be applicable to other enveloped viruses. MDPI 2018-05-16 /pmc/articles/PMC5983600/ /pubmed/29772704 http://dx.doi.org/10.3390/ijms19051483 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Molotkovsky, Rodion J.
Alexandrova, Veronika V.
Galimzyanov, Timur R.
Jiménez-Munguía, Irene
Pavlov, Konstantin V.
Batishchev, Oleg V.
Akimov, Sergey A.
Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title_full Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title_fullStr Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title_full_unstemmed Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title_short Lateral Membrane Heterogeneity Regulates Viral-Induced Membrane Fusion during HIV Entry
title_sort lateral membrane heterogeneity regulates viral-induced membrane fusion during hiv entry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983600/
https://www.ncbi.nlm.nih.gov/pubmed/29772704
http://dx.doi.org/10.3390/ijms19051483
work_keys_str_mv AT molotkovskyrodionj lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT alexandrovaveronikav lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT galimzyanovtimurr lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT jimenezmunguiairene lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT pavlovkonstantinv lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT batishchevolegv lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry
AT akimovsergeya lateralmembraneheterogeneityregulatesviralinducedmembranefusionduringhiventry