Cargando…

MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer

Monocyte chemotactic protein induced protein 3 (MCPIP3) belongs to the Cys–Cys–Cys–His (CCCH)-zinc finger protein family and contains a highly conserved CCCH-zinc finger domain and a Nedd4-BP1 YacP nuclease (NYN) domain. Previous studies showed that MCPIP3 inhibits the expression of proinflammatory...

Descripción completa

Detalles Bibliográficos
Autores principales: Suk, Fat-Moon, Chang, Chi-Ching, Lin, Ren-Jye, Lin, Shyr-Yi, Chen, Ya-Ting, Liang, Yu-Chih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983627/
https://www.ncbi.nlm.nih.gov/pubmed/29751537
http://dx.doi.org/10.3390/ijms19051350
_version_ 1783328461541081088
author Suk, Fat-Moon
Chang, Chi-Ching
Lin, Ren-Jye
Lin, Shyr-Yi
Chen, Ya-Ting
Liang, Yu-Chih
author_facet Suk, Fat-Moon
Chang, Chi-Ching
Lin, Ren-Jye
Lin, Shyr-Yi
Chen, Ya-Ting
Liang, Yu-Chih
author_sort Suk, Fat-Moon
collection PubMed
description Monocyte chemotactic protein induced protein 3 (MCPIP3) belongs to the Cys–Cys–Cys–His (CCCH)-zinc finger protein family and contains a highly conserved CCCH-zinc finger domain and a Nedd4-BP1 YacP nuclease (NYN) domain. Previous studies showed that MCPIP3 inhibits the expression of proinflammatory genes, such as vascular cell adhesion molecule (VCAM)-1, in human endothelial cells, but the roles and functions of MCPIP3 in cancer cells are still unknown. In human colorectal cancer specimens, we found that the messenger RNA expression of MCPIP3 was significantly downregulated in cancer tissues compared to adjacent normal tissues (18/25; average fold change of 8.18). Two cell models were used to demonstrate the anti-migration activity of MCPIP3. First, Tet-on T-REx-293/HA-MCPIP3 cells were used to examine whether MCPIP3 can change epithelial–mesenchymal transition (EMT)-related gene expressions. Second, we used two human colorectal cancer cell lines, SW620 and HCT116, to prove the role of MCPIP3 in regulating EMT-related gene expressions. We found that overexpression of MCPIP3 inhibited cell migration according to a wound-healing assay and Transwell invasion assay and vimentin expression, and increased E-cadherin expression in these two cell lines. These results suggest that MCPIP3 might play a negative role in cell migration of human colorectal cancer cells.
format Online
Article
Text
id pubmed-5983627
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59836272018-06-05 MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer Suk, Fat-Moon Chang, Chi-Ching Lin, Ren-Jye Lin, Shyr-Yi Chen, Ya-Ting Liang, Yu-Chih Int J Mol Sci ijms-19-01350Article Monocyte chemotactic protein induced protein 3 (MCPIP3) belongs to the Cys–Cys–Cys–His (CCCH)-zinc finger protein family and contains a highly conserved CCCH-zinc finger domain and a Nedd4-BP1 YacP nuclease (NYN) domain. Previous studies showed that MCPIP3 inhibits the expression of proinflammatory genes, such as vascular cell adhesion molecule (VCAM)-1, in human endothelial cells, but the roles and functions of MCPIP3 in cancer cells are still unknown. In human colorectal cancer specimens, we found that the messenger RNA expression of MCPIP3 was significantly downregulated in cancer tissues compared to adjacent normal tissues (18/25; average fold change of 8.18). Two cell models were used to demonstrate the anti-migration activity of MCPIP3. First, Tet-on T-REx-293/HA-MCPIP3 cells were used to examine whether MCPIP3 can change epithelial–mesenchymal transition (EMT)-related gene expressions. Second, we used two human colorectal cancer cell lines, SW620 and HCT116, to prove the role of MCPIP3 in regulating EMT-related gene expressions. We found that overexpression of MCPIP3 inhibited cell migration according to a wound-healing assay and Transwell invasion assay and vimentin expression, and increased E-cadherin expression in these two cell lines. These results suggest that MCPIP3 might play a negative role in cell migration of human colorectal cancer cells. MDPI 2018-05-03 /pmc/articles/PMC5983627/ /pubmed/29751537 http://dx.doi.org/10.3390/ijms19051350 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle ijms-19-01350Article
Suk, Fat-Moon
Chang, Chi-Ching
Lin, Ren-Jye
Lin, Shyr-Yi
Chen, Ya-Ting
Liang, Yu-Chih
MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title_full MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title_fullStr MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title_full_unstemmed MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title_short MCPIP3 as a Potential Metastasis Suppressor Gene in Human Colorectal Cancer
title_sort mcpip3 as a potential metastasis suppressor gene in human colorectal cancer
topic ijms-19-01350Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983627/
https://www.ncbi.nlm.nih.gov/pubmed/29751537
http://dx.doi.org/10.3390/ijms19051350
work_keys_str_mv AT sukfatmoon mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer
AT changchiching mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer
AT linrenjye mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer
AT linshyryi mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer
AT chenyating mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer
AT liangyuchih mcpip3asapotentialmetastasissuppressorgeneinhumancolorectalcancer