Cargando…
Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital
Immune-modulating therapy is a promising therapy for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be a favorable predictor for treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim of the current study was to determine the p...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983652/ https://www.ncbi.nlm.nih.gov/pubmed/29747443 http://dx.doi.org/10.3390/ijms19051421 |
_version_ | 1783328467486507008 |
---|---|
author | Winkelmann, Ria Schneider, Markus Hartmann, Sylvia Schnitzbauer, Andreas A. Zeuzem, Stefan Peveling-Oberhag, Jan Hansmann, Martin Leo Walter, Dirk |
author_facet | Winkelmann, Ria Schneider, Markus Hartmann, Sylvia Schnitzbauer, Andreas A. Zeuzem, Stefan Peveling-Oberhag, Jan Hansmann, Martin Leo Walter, Dirk |
author_sort | Winkelmann, Ria |
collection | PubMed |
description | Immune-modulating therapy is a promising therapy for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be a favorable predictor for treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim of the current study was to determine the prevalence of MSI in a German tertiary care hospital. Formalin-fixed paraffin-embedded tissue samples, obtained in the study period from 2007 to 2015 from patients with CCA undergoing surgical resection with curative intention at Johann Wolfgang Goethe University hospital, were examined. All samples were investigated immunohistochemically for the presence of MSI (expression of MLH1, PMS2, MSH2, and MSH6) as well as by pentaplex polymerase chain reaction for five quasimonomorphic mononucleotide repeats (BAT-25, BAT-26, NR-21, NR-22, and NR-24). In total, 102 patients were included, presenting intrahepatic (n = 35, 34.3%), perihilar (n = 42, 41.2%), and distal CCA (n = 25, 24.5%). In the immunohistochemical analysis, no loss of expression of DNA repair enzymes was observed. In the PCR-based analysis, one out of 102 patients was found to be MSI-high and one out of 102 was found to be MSI-low. Thus, MSI seems to appear rarely in CCA in Germany. This should be considered when planning immune-modulating therapy trials for patients with CCA. |
format | Online Article Text |
id | pubmed-5983652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59836522018-06-05 Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital Winkelmann, Ria Schneider, Markus Hartmann, Sylvia Schnitzbauer, Andreas A. Zeuzem, Stefan Peveling-Oberhag, Jan Hansmann, Martin Leo Walter, Dirk Int J Mol Sci Communication Immune-modulating therapy is a promising therapy for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be a favorable predictor for treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim of the current study was to determine the prevalence of MSI in a German tertiary care hospital. Formalin-fixed paraffin-embedded tissue samples, obtained in the study period from 2007 to 2015 from patients with CCA undergoing surgical resection with curative intention at Johann Wolfgang Goethe University hospital, were examined. All samples were investigated immunohistochemically for the presence of MSI (expression of MLH1, PMS2, MSH2, and MSH6) as well as by pentaplex polymerase chain reaction for five quasimonomorphic mononucleotide repeats (BAT-25, BAT-26, NR-21, NR-22, and NR-24). In total, 102 patients were included, presenting intrahepatic (n = 35, 34.3%), perihilar (n = 42, 41.2%), and distal CCA (n = 25, 24.5%). In the immunohistochemical analysis, no loss of expression of DNA repair enzymes was observed. In the PCR-based analysis, one out of 102 patients was found to be MSI-high and one out of 102 was found to be MSI-low. Thus, MSI seems to appear rarely in CCA in Germany. This should be considered when planning immune-modulating therapy trials for patients with CCA. MDPI 2018-05-09 /pmc/articles/PMC5983652/ /pubmed/29747443 http://dx.doi.org/10.3390/ijms19051421 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Winkelmann, Ria Schneider, Markus Hartmann, Sylvia Schnitzbauer, Andreas A. Zeuzem, Stefan Peveling-Oberhag, Jan Hansmann, Martin Leo Walter, Dirk Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title | Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title_full | Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title_fullStr | Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title_full_unstemmed | Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title_short | Microsatellite Instability Occurs Rarely in Patients with Cholangiocarcinoma: A Retrospective Study from a German Tertiary Care Hospital |
title_sort | microsatellite instability occurs rarely in patients with cholangiocarcinoma: a retrospective study from a german tertiary care hospital |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983652/ https://www.ncbi.nlm.nih.gov/pubmed/29747443 http://dx.doi.org/10.3390/ijms19051421 |
work_keys_str_mv | AT winkelmannria microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT schneidermarkus microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT hartmannsylvia microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT schnitzbauerandreasa microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT zeuzemstefan microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT pevelingoberhagjan microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT hansmannmartinleo microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital AT walterdirk microsatelliteinstabilityoccursrarelyinpatientswithcholangiocarcinomaaretrospectivestudyfromagermantertiarycarehospital |