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Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage

The aim of this study is to identify gene expression profiles associated with hyaluronic acid (HA) treatment of normal and osteoarthritis (OA)-like tissue-engineered cartilage. 3D cartilage micromasses were treated with tumour necrosis factor-α (TNF-α) (OA-inducer) and/or HA for 7 days. Viability wa...

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Autores principales: Hemmati-Sadeghi, Shabnam, Ringe, Jochen, Dehne, Tilo, Haag, Rainer, Sittinger, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983669/
https://www.ncbi.nlm.nih.gov/pubmed/29783732
http://dx.doi.org/10.3390/ijms19051519
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author Hemmati-Sadeghi, Shabnam
Ringe, Jochen
Dehne, Tilo
Haag, Rainer
Sittinger, Michael
author_facet Hemmati-Sadeghi, Shabnam
Ringe, Jochen
Dehne, Tilo
Haag, Rainer
Sittinger, Michael
author_sort Hemmati-Sadeghi, Shabnam
collection PubMed
description The aim of this study is to identify gene expression profiles associated with hyaluronic acid (HA) treatment of normal and osteoarthritis (OA)-like tissue-engineered cartilage. 3D cartilage micromasses were treated with tumour necrosis factor-α (TNF-α) (OA-inducer) and/or HA for 7 days. Viability was examined by PI/FDA staining. To document extracellular matrix (ECM) formation, glycosaminoglycans (GAG) were stained with Safranin-O and cartilage-specific type II collagen was detected immunohistochemically. Genome-wide gene expression was determined using microarray analysis. Normal and OA-like micromasses remained vital and showed a spherical morphology and homogenous cell distribution regardless of the treatment. There was no distinct difference in immunolabeling for type II collagen. Safranin-O staining demonstrated a typical depletion of GAG in TNF-α-treated micromasses (−73%), although the extent was limited in the presence of HA (−39%). The microarray data showed that HA can influence the cartilage metabolism via upregulation of TIMP3 in OA-like condition. The upregulation of VEGFA and ANKRD37 genes implies a supportive role of HA in cartilage maturation and survival. The results of this study validate the feasibility of the in vitro OA model for the investigation of HA. On the cellular level, no inhibiting or activating effect of HA was shown. Microarray data demonstrated a minor impact of HA on gene expression level.
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spelling pubmed-59836692018-06-05 Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage Hemmati-Sadeghi, Shabnam Ringe, Jochen Dehne, Tilo Haag, Rainer Sittinger, Michael Int J Mol Sci Article The aim of this study is to identify gene expression profiles associated with hyaluronic acid (HA) treatment of normal and osteoarthritis (OA)-like tissue-engineered cartilage. 3D cartilage micromasses were treated with tumour necrosis factor-α (TNF-α) (OA-inducer) and/or HA for 7 days. Viability was examined by PI/FDA staining. To document extracellular matrix (ECM) formation, glycosaminoglycans (GAG) were stained with Safranin-O and cartilage-specific type II collagen was detected immunohistochemically. Genome-wide gene expression was determined using microarray analysis. Normal and OA-like micromasses remained vital and showed a spherical morphology and homogenous cell distribution regardless of the treatment. There was no distinct difference in immunolabeling for type II collagen. Safranin-O staining demonstrated a typical depletion of GAG in TNF-α-treated micromasses (−73%), although the extent was limited in the presence of HA (−39%). The microarray data showed that HA can influence the cartilage metabolism via upregulation of TIMP3 in OA-like condition. The upregulation of VEGFA and ANKRD37 genes implies a supportive role of HA in cartilage maturation and survival. The results of this study validate the feasibility of the in vitro OA model for the investigation of HA. On the cellular level, no inhibiting or activating effect of HA was shown. Microarray data demonstrated a minor impact of HA on gene expression level. MDPI 2018-05-19 /pmc/articles/PMC5983669/ /pubmed/29783732 http://dx.doi.org/10.3390/ijms19051519 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hemmati-Sadeghi, Shabnam
Ringe, Jochen
Dehne, Tilo
Haag, Rainer
Sittinger, Michael
Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title_full Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title_fullStr Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title_full_unstemmed Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title_short Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
title_sort hyaluronic acid influence on normal and osteoarthritic tissue-engineered cartilage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983669/
https://www.ncbi.nlm.nih.gov/pubmed/29783732
http://dx.doi.org/10.3390/ijms19051519
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