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Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells

Caffeic acid phenethyl ester (CAPE), a bioactive component extracted from propolis, is widely studied due to its anti-cancer effect. Nasopharyngeal carcinoma (NPC) is distinct from other head and neck carcinomas and has a high risk of distant metastases. N-myc downstream regulated gene 1 (NDRG1) is...

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Autores principales: Chiang, Kun-Chun, Yang, Shih-Wei, Chang, Kai-Ping, Feng, Tsui-Hsia, Chang, Kang-Shuo, Tsui, Ke-Hung, Shin, Yi-Syuan, Chen, Chiu-Chun, Chao, Mei, Juang, Horng-Heng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983775/
https://www.ncbi.nlm.nih.gov/pubmed/29738439
http://dx.doi.org/10.3390/ijms19051397
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author Chiang, Kun-Chun
Yang, Shih-Wei
Chang, Kai-Ping
Feng, Tsui-Hsia
Chang, Kang-Shuo
Tsui, Ke-Hung
Shin, Yi-Syuan
Chen, Chiu-Chun
Chao, Mei
Juang, Horng-Heng
author_facet Chiang, Kun-Chun
Yang, Shih-Wei
Chang, Kai-Ping
Feng, Tsui-Hsia
Chang, Kang-Shuo
Tsui, Ke-Hung
Shin, Yi-Syuan
Chen, Chiu-Chun
Chao, Mei
Juang, Horng-Heng
author_sort Chiang, Kun-Chun
collection PubMed
description Caffeic acid phenethyl ester (CAPE), a bioactive component extracted from propolis, is widely studied due to its anti-cancer effect. Nasopharyngeal carcinoma (NPC) is distinct from other head and neck carcinomas and has a high risk of distant metastases. N-myc downstream regulated gene 1 (NDRG1) is demonstrated as a tumor suppressor gene in several cancers. Our result showed that CAPE treatment could repress NPC cell growth, through induction of S phase cell cycle arrest, and invasion. CAPE treatment stimulated NDRG1 expression in NPC cells. NDRG1 knockdown increased NPC cell proliferation and invasion and rendered NPC cells less responsive to CAPE growth-inhibiting effect, indicating CAPE repressed NPC cell growth partly through NDRG1indcution. CAPE treatment increased phosphorylation of ERK, JNK, and p38 in a dose- and time-dependent manner. Pre-treatments by inhibitors of ERK (PD0325901), JNK (SP600125), or p38 (SB201290), respectively, all could partly inhibit the CAPE effect on NDRG1 induction in NPC cells. Further, STAT3 activity was also repressed by CAPE in NPC cells. In summary, CAPE attenuates NPC cell proliferation and invasion by upregulating NDRG1 expression via MAPK pathway and by inhibiting phosphorylation of STAT3. Considering the poor prognosis of NPC patients with metastasis, CAPE could be a promising agent against NPC.
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spelling pubmed-59837752018-06-05 Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells Chiang, Kun-Chun Yang, Shih-Wei Chang, Kai-Ping Feng, Tsui-Hsia Chang, Kang-Shuo Tsui, Ke-Hung Shin, Yi-Syuan Chen, Chiu-Chun Chao, Mei Juang, Horng-Heng Int J Mol Sci Article Caffeic acid phenethyl ester (CAPE), a bioactive component extracted from propolis, is widely studied due to its anti-cancer effect. Nasopharyngeal carcinoma (NPC) is distinct from other head and neck carcinomas and has a high risk of distant metastases. N-myc downstream regulated gene 1 (NDRG1) is demonstrated as a tumor suppressor gene in several cancers. Our result showed that CAPE treatment could repress NPC cell growth, through induction of S phase cell cycle arrest, and invasion. CAPE treatment stimulated NDRG1 expression in NPC cells. NDRG1 knockdown increased NPC cell proliferation and invasion and rendered NPC cells less responsive to CAPE growth-inhibiting effect, indicating CAPE repressed NPC cell growth partly through NDRG1indcution. CAPE treatment increased phosphorylation of ERK, JNK, and p38 in a dose- and time-dependent manner. Pre-treatments by inhibitors of ERK (PD0325901), JNK (SP600125), or p38 (SB201290), respectively, all could partly inhibit the CAPE effect on NDRG1 induction in NPC cells. Further, STAT3 activity was also repressed by CAPE in NPC cells. In summary, CAPE attenuates NPC cell proliferation and invasion by upregulating NDRG1 expression via MAPK pathway and by inhibiting phosphorylation of STAT3. Considering the poor prognosis of NPC patients with metastasis, CAPE could be a promising agent against NPC. MDPI 2018-05-08 /pmc/articles/PMC5983775/ /pubmed/29738439 http://dx.doi.org/10.3390/ijms19051397 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiang, Kun-Chun
Yang, Shih-Wei
Chang, Kai-Ping
Feng, Tsui-Hsia
Chang, Kang-Shuo
Tsui, Ke-Hung
Shin, Yi-Syuan
Chen, Chiu-Chun
Chao, Mei
Juang, Horng-Heng
Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title_full Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title_fullStr Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title_full_unstemmed Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title_short Caffeic Acid Phenethyl Ester Induces N-myc Downstream Regulated Gene 1 to Inhibit Cell Proliferation and Invasion of Human Nasopharyngeal Cancer Cells
title_sort caffeic acid phenethyl ester induces n-myc downstream regulated gene 1 to inhibit cell proliferation and invasion of human nasopharyngeal cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983775/
https://www.ncbi.nlm.nih.gov/pubmed/29738439
http://dx.doi.org/10.3390/ijms19051397
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