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Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava

Cassava, an important food and industrial crop globally, is characterized by its powerful starch accumulation in its storage root. However, the underlying molecular mechanism for this feature remains unclear. Sucrose synthase initializes the conversion of sucrose to starch, and, to a certain extent,...

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Autores principales: Liu, Chen, Chen, Xin, Ma, Ping’an, Zhang, Shengkui, Zeng, Changying, Jiang, Xingyu, Wang, Wenquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983797/
https://www.ncbi.nlm.nih.gov/pubmed/29693589
http://dx.doi.org/10.3390/ijms19051281
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author Liu, Chen
Chen, Xin
Ma, Ping’an
Zhang, Shengkui
Zeng, Changying
Jiang, Xingyu
Wang, Wenquan
author_facet Liu, Chen
Chen, Xin
Ma, Ping’an
Zhang, Shengkui
Zeng, Changying
Jiang, Xingyu
Wang, Wenquan
author_sort Liu, Chen
collection PubMed
description Cassava, an important food and industrial crop globally, is characterized by its powerful starch accumulation in its storage root. However, the underlying molecular mechanism for this feature remains unclear. Sucrose synthase initializes the conversion of sucrose to starch, and, to a certain extent, its enzyme activity can represent sink strength. To understand the modulation of MeSus gene family, the relatively high expressed member in storage root, MeSus1, its promoter was used as bait to screen cassava storage root full-length cDNA library through a yeast one-hybrid system. An ethylene responsive factor cDNA, designated as MeERF72 according to its homolog in Arabidopsis, was screened out. The transcript level of MeERF72 was induced by ethylene, drought, and salt treatments and repressed by abscisic acid, Auxin, gibberellin, salicylic acid, and low and high temperatures. The MeERF72 protein has a conserved APETALA2 domain in its N-terminus and an activated domain of 30 amino acids in its C-terminus, can bind to MeSus1 promoter in vitro and in vivo, and represses the promoter activity of MeSus1. MeERF72 is a transcription factor that can negatively regulate the expression level of MeSus1 in cassava.
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spelling pubmed-59837972018-06-05 Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava Liu, Chen Chen, Xin Ma, Ping’an Zhang, Shengkui Zeng, Changying Jiang, Xingyu Wang, Wenquan Int J Mol Sci Article Cassava, an important food and industrial crop globally, is characterized by its powerful starch accumulation in its storage root. However, the underlying molecular mechanism for this feature remains unclear. Sucrose synthase initializes the conversion of sucrose to starch, and, to a certain extent, its enzyme activity can represent sink strength. To understand the modulation of MeSus gene family, the relatively high expressed member in storage root, MeSus1, its promoter was used as bait to screen cassava storage root full-length cDNA library through a yeast one-hybrid system. An ethylene responsive factor cDNA, designated as MeERF72 according to its homolog in Arabidopsis, was screened out. The transcript level of MeERF72 was induced by ethylene, drought, and salt treatments and repressed by abscisic acid, Auxin, gibberellin, salicylic acid, and low and high temperatures. The MeERF72 protein has a conserved APETALA2 domain in its N-terminus and an activated domain of 30 amino acids in its C-terminus, can bind to MeSus1 promoter in vitro and in vivo, and represses the promoter activity of MeSus1. MeERF72 is a transcription factor that can negatively regulate the expression level of MeSus1 in cassava. MDPI 2018-04-25 /pmc/articles/PMC5983797/ /pubmed/29693589 http://dx.doi.org/10.3390/ijms19051281 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Chen
Chen, Xin
Ma, Ping’an
Zhang, Shengkui
Zeng, Changying
Jiang, Xingyu
Wang, Wenquan
Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title_full Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title_fullStr Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title_full_unstemmed Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title_short Ethylene Responsive Factor MeERF72 Negatively Regulates Sucrose synthase 1 Gene in Cassava
title_sort ethylene responsive factor meerf72 negatively regulates sucrose synthase 1 gene in cassava
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983797/
https://www.ncbi.nlm.nih.gov/pubmed/29693589
http://dx.doi.org/10.3390/ijms19051281
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