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Turnover of Glycerolipid Metabolite Pool and Seed Viability

Hydration–dehydration cycles can frequently cause stress to seeds, but can also be used to improve germination. However, the molecular basis of the stress caused is poorly understood. Herein, we examine the effects of hydration–dehydration cycles on seed viability and profile the membrane glycerolip...

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Autores principales: Hu, Xiao-Long, Yu, Xiao-Mei, Chen, Hong-Ying, Li, Wei-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983817/
https://www.ncbi.nlm.nih.gov/pubmed/29747431
http://dx.doi.org/10.3390/ijms19051417
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author Hu, Xiao-Long
Yu, Xiao-Mei
Chen, Hong-Ying
Li, Wei-Qi
author_facet Hu, Xiao-Long
Yu, Xiao-Mei
Chen, Hong-Ying
Li, Wei-Qi
author_sort Hu, Xiao-Long
collection PubMed
description Hydration–dehydration cycles can frequently cause stress to seeds, but can also be used to improve germination. However, the molecular basis of the stress caused is poorly understood. Herein, we examine the effects of hydration–dehydration cycles on seed viability and profile the membrane glycerolipid molecular species. We find that seed viability was not affected during the first two cycles, but significantly decreased as further cycles were applied, until all viability was lost. The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively, but the phosphatidic acid and diacylglycerol abundances changed in the opposite sense, while total glycerolipid contents remained constant. This suggests that during hydration–dehydration cycles, turnover of glycerolipid metabolite pools take place, while no significant lipid synthesis or degradation is involved. As further hydration–dehydration cycles occurred, lipid unsaturation increased, plastidic lipids decreased, and phosphatidylserine acyl chains lengthened. The latter two could be lethal for seeds. Our findings reveal a novel model of membrane lipid changes, and provide new insights into the responses of seeds to hydration–dehydration cycles.
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spelling pubmed-59838172018-06-05 Turnover of Glycerolipid Metabolite Pool and Seed Viability Hu, Xiao-Long Yu, Xiao-Mei Chen, Hong-Ying Li, Wei-Qi Int J Mol Sci Article Hydration–dehydration cycles can frequently cause stress to seeds, but can also be used to improve germination. However, the molecular basis of the stress caused is poorly understood. Herein, we examine the effects of hydration–dehydration cycles on seed viability and profile the membrane glycerolipid molecular species. We find that seed viability was not affected during the first two cycles, but significantly decreased as further cycles were applied, until all viability was lost. The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively, but the phosphatidic acid and diacylglycerol abundances changed in the opposite sense, while total glycerolipid contents remained constant. This suggests that during hydration–dehydration cycles, turnover of glycerolipid metabolite pools take place, while no significant lipid synthesis or degradation is involved. As further hydration–dehydration cycles occurred, lipid unsaturation increased, plastidic lipids decreased, and phosphatidylserine acyl chains lengthened. The latter two could be lethal for seeds. Our findings reveal a novel model of membrane lipid changes, and provide new insights into the responses of seeds to hydration–dehydration cycles. MDPI 2018-05-09 /pmc/articles/PMC5983817/ /pubmed/29747431 http://dx.doi.org/10.3390/ijms19051417 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Xiao-Long
Yu, Xiao-Mei
Chen, Hong-Ying
Li, Wei-Qi
Turnover of Glycerolipid Metabolite Pool and Seed Viability
title Turnover of Glycerolipid Metabolite Pool and Seed Viability
title_full Turnover of Glycerolipid Metabolite Pool and Seed Viability
title_fullStr Turnover of Glycerolipid Metabolite Pool and Seed Viability
title_full_unstemmed Turnover of Glycerolipid Metabolite Pool and Seed Viability
title_short Turnover of Glycerolipid Metabolite Pool and Seed Viability
title_sort turnover of glycerolipid metabolite pool and seed viability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983817/
https://www.ncbi.nlm.nih.gov/pubmed/29747431
http://dx.doi.org/10.3390/ijms19051417
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