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Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three gro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983840/ https://www.ncbi.nlm.nih.gov/pubmed/29710829 http://dx.doi.org/10.3390/ijms19051322 |
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author | Sayed, Mahmoud Matsui, Naoko Hiraishi, Noriko Nikaido, Toru Burrow, Michael F. Tagami, Junji |
author_facet | Sayed, Mahmoud Matsui, Naoko Hiraishi, Noriko Nikaido, Toru Burrow, Michael F. Tagami, Junji |
author_sort | Sayed, Mahmoud |
collection | PubMed |
description | This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three groups: (1) SDF only (control); (2) SDF followed by application of a potassium iodide solution (KI); and (3) SDF mixed with 20% GSH. Half the specimens were exposed to light and the remainder kept in dark conditions (n = 10) Color changes were measured using a spectrophotometer at the following time intervals: before solution application (baseline) and immediately after application, then 3, 6, 24, 48, 72 h, and 7, 10 and 14 days. SEM/EDS analysis was performed on treated enamel and dentin. Statistical analysis was done using a repeated measures ANOVA test. The spectrophotometer results showed that the SDF group exhibited the greatest color changes under both light exposed and dark conditions, while SDF + GSH group was effective in decreasing the color changes in both light and dark conditions. The SDF + KI group showed an insignificant color changes over time. SEM/EDS analysis showed different patterns for the silver crystal formation in each group (SDF, SDF + GSH, and SDF + KI group). It was concluded GSH can effectively minimize color changes after application of SDF, especially on enamel and to a lesser extent on dentin. |
format | Online Article Text |
id | pubmed-5983840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59838402018-06-05 Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride Sayed, Mahmoud Matsui, Naoko Hiraishi, Noriko Nikaido, Toru Burrow, Michael F. Tagami, Junji Int J Mol Sci Article This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three groups: (1) SDF only (control); (2) SDF followed by application of a potassium iodide solution (KI); and (3) SDF mixed with 20% GSH. Half the specimens were exposed to light and the remainder kept in dark conditions (n = 10) Color changes were measured using a spectrophotometer at the following time intervals: before solution application (baseline) and immediately after application, then 3, 6, 24, 48, 72 h, and 7, 10 and 14 days. SEM/EDS analysis was performed on treated enamel and dentin. Statistical analysis was done using a repeated measures ANOVA test. The spectrophotometer results showed that the SDF group exhibited the greatest color changes under both light exposed and dark conditions, while SDF + GSH group was effective in decreasing the color changes in both light and dark conditions. The SDF + KI group showed an insignificant color changes over time. SEM/EDS analysis showed different patterns for the silver crystal formation in each group (SDF, SDF + GSH, and SDF + KI group). It was concluded GSH can effectively minimize color changes after application of SDF, especially on enamel and to a lesser extent on dentin. MDPI 2018-04-29 /pmc/articles/PMC5983840/ /pubmed/29710829 http://dx.doi.org/10.3390/ijms19051322 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sayed, Mahmoud Matsui, Naoko Hiraishi, Noriko Nikaido, Toru Burrow, Michael F. Tagami, Junji Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title | Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title_full | Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title_fullStr | Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title_full_unstemmed | Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title_short | Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride |
title_sort | effect of glutathione bio-molecule on tooth discoloration associated with silver diammine fluoride |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983840/ https://www.ncbi.nlm.nih.gov/pubmed/29710829 http://dx.doi.org/10.3390/ijms19051322 |
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