Cargando…

Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride

This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three gro...

Descripción completa

Detalles Bibliográficos
Autores principales: Sayed, Mahmoud, Matsui, Naoko, Hiraishi, Noriko, Nikaido, Toru, Burrow, Michael F., Tagami, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983840/
https://www.ncbi.nlm.nih.gov/pubmed/29710829
http://dx.doi.org/10.3390/ijms19051322
_version_ 1783328511902089216
author Sayed, Mahmoud
Matsui, Naoko
Hiraishi, Noriko
Nikaido, Toru
Burrow, Michael F.
Tagami, Junji
author_facet Sayed, Mahmoud
Matsui, Naoko
Hiraishi, Noriko
Nikaido, Toru
Burrow, Michael F.
Tagami, Junji
author_sort Sayed, Mahmoud
collection PubMed
description This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three groups: (1) SDF only (control); (2) SDF followed by application of a potassium iodide solution (KI); and (3) SDF mixed with 20% GSH. Half the specimens were exposed to light and the remainder kept in dark conditions (n = 10) Color changes were measured using a spectrophotometer at the following time intervals: before solution application (baseline) and immediately after application, then 3, 6, 24, 48, 72 h, and 7, 10 and 14 days. SEM/EDS analysis was performed on treated enamel and dentin. Statistical analysis was done using a repeated measures ANOVA test. The spectrophotometer results showed that the SDF group exhibited the greatest color changes under both light exposed and dark conditions, while SDF + GSH group was effective in decreasing the color changes in both light and dark conditions. The SDF + KI group showed an insignificant color changes over time. SEM/EDS analysis showed different patterns for the silver crystal formation in each group (SDF, SDF + GSH, and SDF + KI group). It was concluded GSH can effectively minimize color changes after application of SDF, especially on enamel and to a lesser extent on dentin.
format Online
Article
Text
id pubmed-5983840
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59838402018-06-05 Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride Sayed, Mahmoud Matsui, Naoko Hiraishi, Noriko Nikaido, Toru Burrow, Michael F. Tagami, Junji Int J Mol Sci Article This study evaluated the effect of Glutathione (GSH) bio-molecule on the reduction of enamel and dentin discoloration after application of 38% silver diammine fluoride solution (SDF). One hundred and twenty bovine teeth specimens were used. The enamel and dentin specimens were divided into three groups: (1) SDF only (control); (2) SDF followed by application of a potassium iodide solution (KI); and (3) SDF mixed with 20% GSH. Half the specimens were exposed to light and the remainder kept in dark conditions (n = 10) Color changes were measured using a spectrophotometer at the following time intervals: before solution application (baseline) and immediately after application, then 3, 6, 24, 48, 72 h, and 7, 10 and 14 days. SEM/EDS analysis was performed on treated enamel and dentin. Statistical analysis was done using a repeated measures ANOVA test. The spectrophotometer results showed that the SDF group exhibited the greatest color changes under both light exposed and dark conditions, while SDF + GSH group was effective in decreasing the color changes in both light and dark conditions. The SDF + KI group showed an insignificant color changes over time. SEM/EDS analysis showed different patterns for the silver crystal formation in each group (SDF, SDF + GSH, and SDF + KI group). It was concluded GSH can effectively minimize color changes after application of SDF, especially on enamel and to a lesser extent on dentin. MDPI 2018-04-29 /pmc/articles/PMC5983840/ /pubmed/29710829 http://dx.doi.org/10.3390/ijms19051322 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sayed, Mahmoud
Matsui, Naoko
Hiraishi, Noriko
Nikaido, Toru
Burrow, Michael F.
Tagami, Junji
Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title_full Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title_fullStr Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title_full_unstemmed Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title_short Effect of Glutathione Bio-Molecule on Tooth Discoloration Associated with Silver Diammine Fluoride
title_sort effect of glutathione bio-molecule on tooth discoloration associated with silver diammine fluoride
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983840/
https://www.ncbi.nlm.nih.gov/pubmed/29710829
http://dx.doi.org/10.3390/ijms19051322
work_keys_str_mv AT sayedmahmoud effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride
AT matsuinaoko effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride
AT hiraishinoriko effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride
AT nikaidotoru effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride
AT burrowmichaelf effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride
AT tagamijunji effectofglutathionebiomoleculeontoothdiscolorationassociatedwithsilverdiamminefluoride