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Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery

From a clinical point of view, easily obtainable and useful markers of a particular pathological status are required for appropriate diagnosis and treatment. Analysis of the proteomic profile of saliva may allow for the selection of potential marker of preterm delivery in humans. Saliva samples were...

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Autores principales: Łopucki, Maciej, Wawrzykowski, Jacek, Gęca, Tomasz, Miturski, Andrzej, Franczyk, Monika, Kankofer, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983998/
https://www.ncbi.nlm.nih.gov/pubmed/29693144
http://dx.doi.org/10.3892/mmr.2018.8909
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author Łopucki, Maciej
Wawrzykowski, Jacek
Gęca, Tomasz
Miturski, Andrzej
Franczyk, Monika
Kankofer, Marta
author_facet Łopucki, Maciej
Wawrzykowski, Jacek
Gęca, Tomasz
Miturski, Andrzej
Franczyk, Monika
Kankofer, Marta
author_sort Łopucki, Maciej
collection PubMed
description From a clinical point of view, easily obtainable and useful markers of a particular pathological status are required for appropriate diagnosis and treatment. Analysis of the proteomic profile of saliva may allow for the selection of potential marker of preterm delivery in humans. Saliva samples were collected from 12 patients diagnosed with threatened preterm delivery and 10 controls with uncomplicated pregnancies at the same gestational age. Samples were analysed using 2D electrophoresis. Based on statistical analysis, spots of interest were selected and collected from gels. Subsequently, spots were decoloured and proteins were identified by mass spectrometry using the matrix assisted laser desorption ionization-time of flight technique. The results of identification were compared with the Swiss-Prot database. A total of 1,393 spots were detected in the present study with 59 significantly different between control and preterm samples. Increased intensity of staining of 32 spots was observed in the premature delivery group compared with control patients and 27 spots were stained more intensely in the control group compared with the premature delivery group. A total of nine spots, which were significantly different between examined samples were identified and three of them exhibited increased intensity of staining in premature delivery group compared with controls, including dedicator of cytokinesis protein 1, metallothionein-2, guanylyl cyclase-activating protein 1. The six remaining spots included, epithelial-stromal interaction protein 1, serum albumin, tyrosine-tRNA ligase, cytoplasmic, protein chibby homolog 3, leukemia inhibitory factor receptor and adenosylhomocysteinase 3, and exhibited increased intensity of staining in healthy controls compared with premature delivery group. Further studies with an increased number of patients and identification of the complete protein profile are required to confirm the results of the present study and applicability of saliva as a source of disease biomarkers.
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spelling pubmed-59839982018-06-04 Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery Łopucki, Maciej Wawrzykowski, Jacek Gęca, Tomasz Miturski, Andrzej Franczyk, Monika Kankofer, Marta Mol Med Rep Articles From a clinical point of view, easily obtainable and useful markers of a particular pathological status are required for appropriate diagnosis and treatment. Analysis of the proteomic profile of saliva may allow for the selection of potential marker of preterm delivery in humans. Saliva samples were collected from 12 patients diagnosed with threatened preterm delivery and 10 controls with uncomplicated pregnancies at the same gestational age. Samples were analysed using 2D electrophoresis. Based on statistical analysis, spots of interest were selected and collected from gels. Subsequently, spots were decoloured and proteins were identified by mass spectrometry using the matrix assisted laser desorption ionization-time of flight technique. The results of identification were compared with the Swiss-Prot database. A total of 1,393 spots were detected in the present study with 59 significantly different between control and preterm samples. Increased intensity of staining of 32 spots was observed in the premature delivery group compared with control patients and 27 spots were stained more intensely in the control group compared with the premature delivery group. A total of nine spots, which were significantly different between examined samples were identified and three of them exhibited increased intensity of staining in premature delivery group compared with controls, including dedicator of cytokinesis protein 1, metallothionein-2, guanylyl cyclase-activating protein 1. The six remaining spots included, epithelial-stromal interaction protein 1, serum albumin, tyrosine-tRNA ligase, cytoplasmic, protein chibby homolog 3, leukemia inhibitory factor receptor and adenosylhomocysteinase 3, and exhibited increased intensity of staining in healthy controls compared with premature delivery group. Further studies with an increased number of patients and identification of the complete protein profile are required to confirm the results of the present study and applicability of saliva as a source of disease biomarkers. D.A. Spandidos 2018-06 2018-04-20 /pmc/articles/PMC5983998/ /pubmed/29693144 http://dx.doi.org/10.3892/mmr.2018.8909 Text en Copyright: © Łopucki et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Łopucki, Maciej
Wawrzykowski, Jacek
Gęca, Tomasz
Miturski, Andrzej
Franczyk, Monika
Kankofer, Marta
Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title_full Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title_fullStr Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title_full_unstemmed Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title_short Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
title_sort preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983998/
https://www.ncbi.nlm.nih.gov/pubmed/29693144
http://dx.doi.org/10.3892/mmr.2018.8909
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