Cargando…

PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits

The present study aimed to investigate whether bone morphogenetic protein-2 (BMP-2) was involved in the repair of mandibular defects using polyether-ether-ketone biphasic bioceramic (PEEK-BBC) composites in rabbits. PEEK-BBC composites with abundant and interconnected pores were prepared by calcinat...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Hedong, Chen, Yongji, Mao, Min, Liu, Dongyan, Ai, Jun, Leng, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983999/
https://www.ncbi.nlm.nih.gov/pubmed/29658566
http://dx.doi.org/10.3892/mmr.2018.8867
_version_ 1783328542817255424
author Yu, Hedong
Chen, Yongji
Mao, Min
Liu, Dongyan
Ai, Jun
Leng, Weidong
author_facet Yu, Hedong
Chen, Yongji
Mao, Min
Liu, Dongyan
Ai, Jun
Leng, Weidong
author_sort Yu, Hedong
collection PubMed
description The present study aimed to investigate whether bone morphogenetic protein-2 (BMP-2) was involved in the repair of mandibular defects using polyether-ether-ketone biphasic bioceramic (PEEK-BBC) composites in rabbits. PEEK-BBC composites with abundant and interconnected pores were prepared by calcination and characterized by scanning electron microscope. A mandibular defect model in rabbits was established using dental grinder to produce a square hole. A total of 60 rabbits were divided into four groups: Control, sham, surgery, and PEEK. In the PEEK group, the holes were filled with the PEEK-BBC composite stents. In the surgery group, the holes were produced but not filled with the composite stents. In the sham group, only the molar grooves were exposed and grinding was not performed. Animals without any treatment served as the control group. The success rate of model establishment was 100%. At 4, 8, and 16 weeks after the model was established, samples were collected from the molding sites. Bone repair was evaluated by H&E staining and Goldner trichrome staining. Bone structures in both control and sham groups were intact. A small number of osteocytes were observed in the surgery group. However, in the PEEK group, osteocytes were already evidently present in the composites at 4 weeks after surgery. At 8 and 16 weeks, there were large numbers of osteocytes in the pores of the composites. The mRNA and protein expression levels of BMP-2 were determined by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The mRNA and protein expression levels of BMP-2 between the control and sham groups were similar and were continuously stable. However, following defect treatment, BMP-2 mRNA and protein expression was upregulated, which was enhanced by the PEEK-BBC composites. In conclusion, PEEK-BBC composites promoted the growth of osteocytes and repaired mandibular defects in rabbits, potentially via the upregulation of BMP-2 expression.
format Online
Article
Text
id pubmed-5983999
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-59839992018-06-04 PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits Yu, Hedong Chen, Yongji Mao, Min Liu, Dongyan Ai, Jun Leng, Weidong Mol Med Rep Articles The present study aimed to investigate whether bone morphogenetic protein-2 (BMP-2) was involved in the repair of mandibular defects using polyether-ether-ketone biphasic bioceramic (PEEK-BBC) composites in rabbits. PEEK-BBC composites with abundant and interconnected pores were prepared by calcination and characterized by scanning electron microscope. A mandibular defect model in rabbits was established using dental grinder to produce a square hole. A total of 60 rabbits were divided into four groups: Control, sham, surgery, and PEEK. In the PEEK group, the holes were filled with the PEEK-BBC composite stents. In the surgery group, the holes were produced but not filled with the composite stents. In the sham group, only the molar grooves were exposed and grinding was not performed. Animals without any treatment served as the control group. The success rate of model establishment was 100%. At 4, 8, and 16 weeks after the model was established, samples were collected from the molding sites. Bone repair was evaluated by H&E staining and Goldner trichrome staining. Bone structures in both control and sham groups were intact. A small number of osteocytes were observed in the surgery group. However, in the PEEK group, osteocytes were already evidently present in the composites at 4 weeks after surgery. At 8 and 16 weeks, there were large numbers of osteocytes in the pores of the composites. The mRNA and protein expression levels of BMP-2 were determined by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The mRNA and protein expression levels of BMP-2 between the control and sham groups were similar and were continuously stable. However, following defect treatment, BMP-2 mRNA and protein expression was upregulated, which was enhanced by the PEEK-BBC composites. In conclusion, PEEK-BBC composites promoted the growth of osteocytes and repaired mandibular defects in rabbits, potentially via the upregulation of BMP-2 expression. D.A. Spandidos 2018-06 2018-04-11 /pmc/articles/PMC5983999/ /pubmed/29658566 http://dx.doi.org/10.3892/mmr.2018.8867 Text en Copyright: © Yu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yu, Hedong
Chen, Yongji
Mao, Min
Liu, Dongyan
Ai, Jun
Leng, Weidong
PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title_full PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title_fullStr PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title_full_unstemmed PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title_short PEEK-biphasic bioceramic composites promote mandibular defect repair and upregulate BMP-2 expression in rabbits
title_sort peek-biphasic bioceramic composites promote mandibular defect repair and upregulate bmp-2 expression in rabbits
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983999/
https://www.ncbi.nlm.nih.gov/pubmed/29658566
http://dx.doi.org/10.3892/mmr.2018.8867
work_keys_str_mv AT yuhedong peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits
AT chenyongji peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits
AT maomin peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits
AT liudongyan peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits
AT aijun peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits
AT lengweidong peekbiphasicbioceramiccompositespromotemandibulardefectrepairandupregulatebmp2expressioninrabbits