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Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth

BACKGROUND AND OBJECTIVES: Stem cells from human exfoliated deciduous teeth (SHED) are a promising clinical resource for various tissue defects, including lumbar spondylosis, neural compression, and cleft palate. Use of media containing animal-derived serum carries potential risk of infectious disea...

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Autores principales: Tangjit, Nathaphon, Dechkunakorn, Surachai, Anuwongnukroh, Niwat, Khaneungthong, Anuson, Sritanaudomchai, Hathaitip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Stem Cell Research 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984063/
https://www.ncbi.nlm.nih.gov/pubmed/29482310
http://dx.doi.org/10.15283/ijsc17049
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author Tangjit, Nathaphon
Dechkunakorn, Surachai
Anuwongnukroh, Niwat
Khaneungthong, Anuson
Sritanaudomchai, Hathaitip
author_facet Tangjit, Nathaphon
Dechkunakorn, Surachai
Anuwongnukroh, Niwat
Khaneungthong, Anuson
Sritanaudomchai, Hathaitip
author_sort Tangjit, Nathaphon
collection PubMed
description BACKGROUND AND OBJECTIVES: Stem cells from human exfoliated deciduous teeth (SHED) are a promising clinical resource for various tissue defects, including lumbar spondylosis, neural compression, and cleft palate. Use of media containing animal-derived serum carries potential risk of infectious diseases and unwanted immunogenicity. To increase the potential utility of SHED for clinical application, SHED was adapted to xeno-free conditions. METHODS: Define xeno-free culture media were compared with the conventional serum containing media in the culture of SHED. Cultured SHED in different media were further characterized through proliferative capacities, cellular phenotype, and differentiation potential. RESULTS: Selected xeno-free media were capable of supporting the growth of SHED. MSCGM-CD Bulletkit medium greatly increased the number and proliferate capacity of colony-forming unit-fibroblast than SHED cultured in other media. In addition, the characteristic surface markers expression and multipotent differentiation potential of SHED in the MSCGM-CD Bulletkit medium were comparable to those observed with serum-containing medium. CONCLUSIONS: The xeno-free medium described herein has the potential to be further used for the safe expansion and to determine efficient way to produce clinical grade dental stem cells for therapeutic applications.
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spelling pubmed-59840632018-06-12 Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth Tangjit, Nathaphon Dechkunakorn, Surachai Anuwongnukroh, Niwat Khaneungthong, Anuson Sritanaudomchai, Hathaitip Int J Stem Cells Original Article BACKGROUND AND OBJECTIVES: Stem cells from human exfoliated deciduous teeth (SHED) are a promising clinical resource for various tissue defects, including lumbar spondylosis, neural compression, and cleft palate. Use of media containing animal-derived serum carries potential risk of infectious diseases and unwanted immunogenicity. To increase the potential utility of SHED for clinical application, SHED was adapted to xeno-free conditions. METHODS: Define xeno-free culture media were compared with the conventional serum containing media in the culture of SHED. Cultured SHED in different media were further characterized through proliferative capacities, cellular phenotype, and differentiation potential. RESULTS: Selected xeno-free media were capable of supporting the growth of SHED. MSCGM-CD Bulletkit medium greatly increased the number and proliferate capacity of colony-forming unit-fibroblast than SHED cultured in other media. In addition, the characteristic surface markers expression and multipotent differentiation potential of SHED in the MSCGM-CD Bulletkit medium were comparable to those observed with serum-containing medium. CONCLUSIONS: The xeno-free medium described herein has the potential to be further used for the safe expansion and to determine efficient way to produce clinical grade dental stem cells for therapeutic applications. Korean Society for Stem Cell Research 2018-02-28 /pmc/articles/PMC5984063/ /pubmed/29482310 http://dx.doi.org/10.15283/ijsc17049 Text en Copyright © 2018 by the Korean Society for Stem Cell Research This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tangjit, Nathaphon
Dechkunakorn, Surachai
Anuwongnukroh, Niwat
Khaneungthong, Anuson
Sritanaudomchai, Hathaitip
Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title_full Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title_fullStr Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title_full_unstemmed Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title_short Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
title_sort optimal xeno-free culture condition for clinical grade stem cells from human exfoliated deciduous teeth
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984063/
https://www.ncbi.nlm.nih.gov/pubmed/29482310
http://dx.doi.org/10.15283/ijsc17049
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