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Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution
BACKGROUND: Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and their act...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984802/ https://www.ncbi.nlm.nih.gov/pubmed/29859069 http://dx.doi.org/10.1186/s12896-018-0451-0 |