Cargando…
Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cel...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984946/ https://www.ncbi.nlm.nih.gov/pubmed/29860613 http://dx.doi.org/10.1186/s13568-018-0624-1 |
_version_ | 1783328685821001728 |
---|---|
author | Xu, Xian Tian, Liqing Xu, Jiali Xie, Chengjia Jiang, Ling Huang, He |
author_facet | Xu, Xian Tian, Liqing Xu, Jiali Xie, Chengjia Jiang, Ling Huang, He |
author_sort | Xu, Xian |
collection | PubMed |
description | Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells’ antioxidant defense, were identified in the sequenced genome of R12 and analyzed. In order to improve the carotenoid yield in engineered Escherichia coli, the origin of carotenoid biosynthesis genes was discussed, and a strain containing the R12 carotenoid biosynthesis genes was constructed to produce lycopene, an important intermediate in carotenoid metabolism. The gene order and fermentation conditions, including the culture medium, temperature, and light, were optimized to obtain a genetically engineered strain with a high lycopene production capacity. The highest lycopene content was 688 mg L(−1) in strain IEB, which corresponds to a 2.2-fold improvement over the original recombinant strain EBI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0624-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5984946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-59849462018-06-14 Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli Xu, Xian Tian, Liqing Xu, Jiali Xie, Chengjia Jiang, Ling Huang, He AMB Express Original Article Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells’ antioxidant defense, were identified in the sequenced genome of R12 and analyzed. In order to improve the carotenoid yield in engineered Escherichia coli, the origin of carotenoid biosynthesis genes was discussed, and a strain containing the R12 carotenoid biosynthesis genes was constructed to produce lycopene, an important intermediate in carotenoid metabolism. The gene order and fermentation conditions, including the culture medium, temperature, and light, were optimized to obtain a genetically engineered strain with a high lycopene production capacity. The highest lycopene content was 688 mg L(−1) in strain IEB, which corresponds to a 2.2-fold improvement over the original recombinant strain EBI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0624-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-06-02 /pmc/articles/PMC5984946/ /pubmed/29860613 http://dx.doi.org/10.1186/s13568-018-0624-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Xu, Xian Tian, Liqing Xu, Jiali Xie, Chengjia Jiang, Ling Huang, He Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title | Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title_full | Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title_fullStr | Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title_full_unstemmed | Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title_short | Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli |
title_sort | analysis and expression of the carotenoid biosynthesis genes from deinococcus wulumuqiensis r12 in engineered escherichia coli |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984946/ https://www.ncbi.nlm.nih.gov/pubmed/29860613 http://dx.doi.org/10.1186/s13568-018-0624-1 |
work_keys_str_mv | AT xuxian analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli AT tianliqing analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli AT xujiali analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli AT xiechengjia analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli AT jiangling analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli AT huanghe analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli |