Cargando…

Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli

Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Xian, Tian, Liqing, Xu, Jiali, Xie, Chengjia, Jiang, Ling, Huang, He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984946/
https://www.ncbi.nlm.nih.gov/pubmed/29860613
http://dx.doi.org/10.1186/s13568-018-0624-1
_version_ 1783328685821001728
author Xu, Xian
Tian, Liqing
Xu, Jiali
Xie, Chengjia
Jiang, Ling
Huang, He
author_facet Xu, Xian
Tian, Liqing
Xu, Jiali
Xie, Chengjia
Jiang, Ling
Huang, He
author_sort Xu, Xian
collection PubMed
description Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells’ antioxidant defense, were identified in the sequenced genome of R12 and analyzed. In order to improve the carotenoid yield in engineered Escherichia coli, the origin of carotenoid biosynthesis genes was discussed, and a strain containing the R12 carotenoid biosynthesis genes was constructed to produce lycopene, an important intermediate in carotenoid metabolism. The gene order and fermentation conditions, including the culture medium, temperature, and light, were optimized to obtain a genetically engineered strain with a high lycopene production capacity. The highest lycopene content was 688 mg L(−1) in strain IEB, which corresponds to a 2.2-fold improvement over the original recombinant strain EBI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0624-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5984946
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-59849462018-06-14 Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli Xu, Xian Tian, Liqing Xu, Jiali Xie, Chengjia Jiang, Ling Huang, He AMB Express Original Article Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells’ antioxidant defense, were identified in the sequenced genome of R12 and analyzed. In order to improve the carotenoid yield in engineered Escherichia coli, the origin of carotenoid biosynthesis genes was discussed, and a strain containing the R12 carotenoid biosynthesis genes was constructed to produce lycopene, an important intermediate in carotenoid metabolism. The gene order and fermentation conditions, including the culture medium, temperature, and light, were optimized to obtain a genetically engineered strain with a high lycopene production capacity. The highest lycopene content was 688 mg L(−1) in strain IEB, which corresponds to a 2.2-fold improvement over the original recombinant strain EBI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0624-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-06-02 /pmc/articles/PMC5984946/ /pubmed/29860613 http://dx.doi.org/10.1186/s13568-018-0624-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Xu, Xian
Tian, Liqing
Xu, Jiali
Xie, Chengjia
Jiang, Ling
Huang, He
Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title_full Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title_fullStr Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title_full_unstemmed Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title_short Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
title_sort analysis and expression of the carotenoid biosynthesis genes from deinococcus wulumuqiensis r12 in engineered escherichia coli
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984946/
https://www.ncbi.nlm.nih.gov/pubmed/29860613
http://dx.doi.org/10.1186/s13568-018-0624-1
work_keys_str_mv AT xuxian analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli
AT tianliqing analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli
AT xujiali analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli
AT xiechengjia analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli
AT jiangling analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli
AT huanghe analysisandexpressionofthecarotenoidbiosynthesisgenesfromdeinococcuswulumuqiensisr12inengineeredescherichiacoli