Cargando…

MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status

Glioma is the most common primary brain tumor with various genetic alterations; among which, IDH mutation is the most common mutation and plays an important role in glioma early development, especially in lower grade glioma (LGG, WHO II-III). Previous studies have found that IDH mutation is tightly...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Guanzhang, Wang, Zhiliang, Zhang, Chuanbao, Liu, Xing, Yang, Fuqiang, Sun, Lihua, Liang, Jingshan, Hu, Huimin, Liu, Yanwei, You, Gan, Bao, Zhaoshi, Zhang, Wei, Wang, Zheng, Jiang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985127/
https://www.ncbi.nlm.nih.gov/pubmed/29887919
http://dx.doi.org/10.1155/2018/5975216
_version_ 1783328711936835584
author Li, Guanzhang
Wang, Zhiliang
Zhang, Chuanbao
Liu, Xing
Yang, Fuqiang
Sun, Lihua
Liang, Jingshan
Hu, Huimin
Liu, Yanwei
You, Gan
Bao, Zhaoshi
Zhang, Wei
Wang, Zheng
Jiang, Tao
author_facet Li, Guanzhang
Wang, Zhiliang
Zhang, Chuanbao
Liu, Xing
Yang, Fuqiang
Sun, Lihua
Liang, Jingshan
Hu, Huimin
Liu, Yanwei
You, Gan
Bao, Zhaoshi
Zhang, Wei
Wang, Zheng
Jiang, Tao
author_sort Li, Guanzhang
collection PubMed
description Glioma is the most common primary brain tumor with various genetic alterations; among which, IDH mutation is the most common mutation and plays an important role in glioma early development, especially in lower grade glioma (LGG, WHO II-III). Previous studies have found that IDH mutation is tightly associated with extensive methylation across whole genome in glioma. To further investigate the role of IDH, we obtained methylation data of 777 samples from CGGA (Chinese Glioma Genome Atlas) and TCGA (The Cancer Genome Atlas) with IDH mutation status available. A package compiled under R language called Tspair was used as the main analytic tool to find potential probes that were significantly affected by IDH mutation. As a result, we found one pair of probes, cg06940792 and cg26025891, which was capable of predicting IDH mutation status precisely. The hypermethylated probe was cg06940792, designed in the promoter region of MEGF10, while the hypomethylated probe was cg26025891, designed in the promoter region of PSTPIP1. Survival analysis proved that hypermethylation or low expression of MEGF10 indicated a favorable prognosis in 983 glioma samples. Moreover, gene ontology analysis demonstrated that MEGF10 was associated with cell migration, cell proliferation, and regulation of apoptosis in glioma. All findings above can be validated in three other independent cohorts. In a word, our results suggested that methylation level and mRNA expression of MEGF10 in glioma were not only correlated with IDH mutation but also associated with clinical outcome of patients, providing potential guide for future dissection of IDH role in glioma.
format Online
Article
Text
id pubmed-5985127
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-59851272018-06-10 MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status Li, Guanzhang Wang, Zhiliang Zhang, Chuanbao Liu, Xing Yang, Fuqiang Sun, Lihua Liang, Jingshan Hu, Huimin Liu, Yanwei You, Gan Bao, Zhaoshi Zhang, Wei Wang, Zheng Jiang, Tao Dis Markers Research Article Glioma is the most common primary brain tumor with various genetic alterations; among which, IDH mutation is the most common mutation and plays an important role in glioma early development, especially in lower grade glioma (LGG, WHO II-III). Previous studies have found that IDH mutation is tightly associated with extensive methylation across whole genome in glioma. To further investigate the role of IDH, we obtained methylation data of 777 samples from CGGA (Chinese Glioma Genome Atlas) and TCGA (The Cancer Genome Atlas) with IDH mutation status available. A package compiled under R language called Tspair was used as the main analytic tool to find potential probes that were significantly affected by IDH mutation. As a result, we found one pair of probes, cg06940792 and cg26025891, which was capable of predicting IDH mutation status precisely. The hypermethylated probe was cg06940792, designed in the promoter region of MEGF10, while the hypomethylated probe was cg26025891, designed in the promoter region of PSTPIP1. Survival analysis proved that hypermethylation or low expression of MEGF10 indicated a favorable prognosis in 983 glioma samples. Moreover, gene ontology analysis demonstrated that MEGF10 was associated with cell migration, cell proliferation, and regulation of apoptosis in glioma. All findings above can be validated in three other independent cohorts. In a word, our results suggested that methylation level and mRNA expression of MEGF10 in glioma were not only correlated with IDH mutation but also associated with clinical outcome of patients, providing potential guide for future dissection of IDH role in glioma. Hindawi 2018-05-20 /pmc/articles/PMC5985127/ /pubmed/29887919 http://dx.doi.org/10.1155/2018/5975216 Text en Copyright © 2018 Guanzhang Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Guanzhang
Wang, Zhiliang
Zhang, Chuanbao
Liu, Xing
Yang, Fuqiang
Sun, Lihua
Liang, Jingshan
Hu, Huimin
Liu, Yanwei
You, Gan
Bao, Zhaoshi
Zhang, Wei
Wang, Zheng
Jiang, Tao
MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title_full MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title_fullStr MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title_full_unstemmed MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title_short MEGF10, a Glioma Survival-Associated Molecular Signature, Predicts IDH Mutation Status
title_sort megf10, a glioma survival-associated molecular signature, predicts idh mutation status
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985127/
https://www.ncbi.nlm.nih.gov/pubmed/29887919
http://dx.doi.org/10.1155/2018/5975216
work_keys_str_mv AT liguanzhang megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT wangzhiliang megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT zhangchuanbao megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT liuxing megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT yangfuqiang megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT sunlihua megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT liangjingshan megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT huhuimin megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT liuyanwei megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT yougan megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT baozhaoshi megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT zhangwei megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT wangzheng megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus
AT jiangtao megf10agliomasurvivalassociatedmolecularsignaturepredictsidhmutationstatus