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Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults

OBJECTIVES: Environmental enteropathy is prevalent in low-income countries, although its aetiology is unknown. We investigated if Mycobacterium avium antigens, which are commonly found in the environment, could contribute to its pathogenesis in a population known to have widespread environmental ent...

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Autores principales: Chongwe, Gershom, Michelo, Charles, Sinkala, Edford, Kayamba, Violet, Nzayisenga, Jean-Baptiste, Drobniewski, Francis, Kelly, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985370/
https://www.ncbi.nlm.nih.gov/pubmed/29680481
http://dx.doi.org/10.1016/j.ijid.2018.03.020
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author Chongwe, Gershom
Michelo, Charles
Sinkala, Edford
Kayamba, Violet
Nzayisenga, Jean-Baptiste
Drobniewski, Francis
Kelly, Paul
author_facet Chongwe, Gershom
Michelo, Charles
Sinkala, Edford
Kayamba, Violet
Nzayisenga, Jean-Baptiste
Drobniewski, Francis
Kelly, Paul
author_sort Chongwe, Gershom
collection PubMed
description OBJECTIVES: Environmental enteropathy is prevalent in low-income countries, although its aetiology is unknown. We investigated if Mycobacterium avium antigens, which are commonly found in the environment, could contribute to its pathogenesis in a population known to have widespread environmental enteropathy. METHODS: Routine endoscopy patients at the University Teaching Hospital, Lusaka whose endoscopy results were normal submitted duodenal biopsies and whole blood samples. Samples were stimulated with M. avium lysate over 24 h while unstimulated samples served as negative controls. Matrix metalloproteinase (MMP) and cytokine response in supernatants were quantified using ELISA and cytometric bead array. RESULTS: Samples from 48 patients (56% women) were analysed, with a median age of 35 years (IQR 27.5, 50.5). M. avium induced the secretion of a wide-range of Th1, Th2 and Th17 cytokines in blood but only IL-1β and IL-6 in duodenal tissue. However it differentially induced the secretion of MMP-1 in duodenal tissue compared to negative controls (p = 0.004). A similar MMP-1 response but with lower concentrations was observed in blood. CONCLUSION: The induction of MMP-1 and cytokines by M. avium in duodenal tissue suggests that environmental mycobacteria could contribute to the epithelial disruption seen in environmental enteropathy, and a need to further explore possible biomarkers that may predict this exposure in at-risk populations.
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spelling pubmed-59853702018-06-05 Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults Chongwe, Gershom Michelo, Charles Sinkala, Edford Kayamba, Violet Nzayisenga, Jean-Baptiste Drobniewski, Francis Kelly, Paul Int J Infect Dis Article OBJECTIVES: Environmental enteropathy is prevalent in low-income countries, although its aetiology is unknown. We investigated if Mycobacterium avium antigens, which are commonly found in the environment, could contribute to its pathogenesis in a population known to have widespread environmental enteropathy. METHODS: Routine endoscopy patients at the University Teaching Hospital, Lusaka whose endoscopy results were normal submitted duodenal biopsies and whole blood samples. Samples were stimulated with M. avium lysate over 24 h while unstimulated samples served as negative controls. Matrix metalloproteinase (MMP) and cytokine response in supernatants were quantified using ELISA and cytometric bead array. RESULTS: Samples from 48 patients (56% women) were analysed, with a median age of 35 years (IQR 27.5, 50.5). M. avium induced the secretion of a wide-range of Th1, Th2 and Th17 cytokines in blood but only IL-1β and IL-6 in duodenal tissue. However it differentially induced the secretion of MMP-1 in duodenal tissue compared to negative controls (p = 0.004). A similar MMP-1 response but with lower concentrations was observed in blood. CONCLUSION: The induction of MMP-1 and cytokines by M. avium in duodenal tissue suggests that environmental mycobacteria could contribute to the epithelial disruption seen in environmental enteropathy, and a need to further explore possible biomarkers that may predict this exposure in at-risk populations. Elsevier 2018-06 /pmc/articles/PMC5985370/ /pubmed/29680481 http://dx.doi.org/10.1016/j.ijid.2018.03.020 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chongwe, Gershom
Michelo, Charles
Sinkala, Edford
Kayamba, Violet
Nzayisenga, Jean-Baptiste
Drobniewski, Francis
Kelly, Paul
Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title_full Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title_fullStr Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title_full_unstemmed Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title_short Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: Observations from selected hospital based Zambian adults
title_sort mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: observations from selected hospital based zambian adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985370/
https://www.ncbi.nlm.nih.gov/pubmed/29680481
http://dx.doi.org/10.1016/j.ijid.2018.03.020
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