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Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy

Assessment of muscle pathology is a key outcome measure to measure the success of clinical trials studying muscular dystrophies; however, few robust minimally invasive measures exist. Indocyanine green (ICG)-enhanced near-infrared (NIR) optical imaging offers an objective, minimally invasive, and lo...

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Autores principales: Chrzanowski, Stephen M., Vohra, Ravneet S., Lee-McMullen, Brittany A., Batra, Abhinandan, Spradlin, Ray A., Morales, Jazmine, Forbes, Sean, Vandenborne, Krista, Barton, Elisabeth R., Walter, Glenn A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985549/
https://www.ncbi.nlm.nih.gov/pubmed/29271299
http://dx.doi.org/10.1177/1536012117732439
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author Chrzanowski, Stephen M.
Vohra, Ravneet S.
Lee-McMullen, Brittany A.
Batra, Abhinandan
Spradlin, Ray A.
Morales, Jazmine
Forbes, Sean
Vandenborne, Krista
Barton, Elisabeth R.
Walter, Glenn A.
author_facet Chrzanowski, Stephen M.
Vohra, Ravneet S.
Lee-McMullen, Brittany A.
Batra, Abhinandan
Spradlin, Ray A.
Morales, Jazmine
Forbes, Sean
Vandenborne, Krista
Barton, Elisabeth R.
Walter, Glenn A.
author_sort Chrzanowski, Stephen M.
collection PubMed
description Assessment of muscle pathology is a key outcome measure to measure the success of clinical trials studying muscular dystrophies; however, few robust minimally invasive measures exist. Indocyanine green (ICG)-enhanced near-infrared (NIR) optical imaging offers an objective, minimally invasive, and longitudinal modality that can quantify pathology within muscle by imaging uptake of ICG into the damaged muscles. Dystrophic mice lacking dystrophin (mdx) or gamma-sarcoglycan (Sgcg(−/−)) were compared to control mice by NIR optical imaging and magnetic resonance imaging (MRI). We determined that optical imaging could be used to differentiate control and dystrophic mice, visualize eccentric muscle induced by downhill treadmill running, and restore the membrane integrity in Sgcg(−/−) mice following adeno-associated virus (AAV) delivery of recombinant human SGCG (desAAV8hSGCG). We conclude that NIR optical imaging is comparable to MRI and can be used to detect muscle damage in dystrophic muscle as compared to unaffected controls, monitor worsening of muscle pathology in muscular dystrophy, and assess regression of pathology following therapeutic intervention in muscular dystrophies.
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spelling pubmed-59855492018-06-13 Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy Chrzanowski, Stephen M. Vohra, Ravneet S. Lee-McMullen, Brittany A. Batra, Abhinandan Spradlin, Ray A. Morales, Jazmine Forbes, Sean Vandenborne, Krista Barton, Elisabeth R. Walter, Glenn A. Mol Imaging Research Article Assessment of muscle pathology is a key outcome measure to measure the success of clinical trials studying muscular dystrophies; however, few robust minimally invasive measures exist. Indocyanine green (ICG)-enhanced near-infrared (NIR) optical imaging offers an objective, minimally invasive, and longitudinal modality that can quantify pathology within muscle by imaging uptake of ICG into the damaged muscles. Dystrophic mice lacking dystrophin (mdx) or gamma-sarcoglycan (Sgcg(−/−)) were compared to control mice by NIR optical imaging and magnetic resonance imaging (MRI). We determined that optical imaging could be used to differentiate control and dystrophic mice, visualize eccentric muscle induced by downhill treadmill running, and restore the membrane integrity in Sgcg(−/−) mice following adeno-associated virus (AAV) delivery of recombinant human SGCG (desAAV8hSGCG). We conclude that NIR optical imaging is comparable to MRI and can be used to detect muscle damage in dystrophic muscle as compared to unaffected controls, monitor worsening of muscle pathology in muscular dystrophy, and assess regression of pathology following therapeutic intervention in muscular dystrophies. SAGE Publications 2017-12-22 /pmc/articles/PMC5985549/ /pubmed/29271299 http://dx.doi.org/10.1177/1536012117732439 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Chrzanowski, Stephen M.
Vohra, Ravneet S.
Lee-McMullen, Brittany A.
Batra, Abhinandan
Spradlin, Ray A.
Morales, Jazmine
Forbes, Sean
Vandenborne, Krista
Barton, Elisabeth R.
Walter, Glenn A.
Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title_full Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title_fullStr Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title_full_unstemmed Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title_short Contrast-Enhanced Near-Infrared Optical Imaging Detects Exacerbation and Amelioration of Murine Muscular Dystrophy
title_sort contrast-enhanced near-infrared optical imaging detects exacerbation and amelioration of murine muscular dystrophy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985549/
https://www.ncbi.nlm.nih.gov/pubmed/29271299
http://dx.doi.org/10.1177/1536012117732439
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