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Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences

The modulation of expression levels of fluorescent fusion proteins (FFPs) is central for recombinant DNA technologies in modern biology as overexpression of proteins contributes to artifacts in biological experiments. In addition, some microscopy techniques such as fluorescence correlation spectrosc...

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Detalles Bibliográficos
Autores principales: Ali, Rizwan, Ramadurai, Sivaramakrishnan, Barry, Frank, Nasheuer, Heinz Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985997/
https://www.ncbi.nlm.nih.gov/pubmed/29928582
http://dx.doi.org/10.1002/2211-5463.12432

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