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Proteomic Analysis of the Cell Cycle of Procylic Form Trypanosoma brucei

We describe a single-step centrifugal elutriation method to produce synchronous Gap1 (G1)-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag (TMT) labeling and mass spectrometry (MS)-based proteomics technology, the expression le...

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Detalles Bibliográficos
Autores principales:	Crozier, Thomas W. M., Tinti, Michele, Wheeler, Richard J., Ly, Tony, Ferguson, Michael A. J., Lamond, Angus I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The American Society for Biochemistry and Molecular Biology 2018
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986242/ https://www.ncbi.nlm.nih.gov/pubmed/29555687 http://dx.doi.org/10.1074/mcp.RA118.000650

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