Cargando…

In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract

The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis p...

Descripción completa

Detalles Bibliográficos
Autores principales: Vamanu, Emanuel, Ene, Mihaela, Biță, Bogdan, Ionescu, Cristina, Crăciun, Liviu, Sârbu, Ionela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986487/
https://www.ncbi.nlm.nih.gov/pubmed/29757931
http://dx.doi.org/10.3390/nu10050607
_version_ 1783328934738264064
author Vamanu, Emanuel
Ene, Mihaela
Biță, Bogdan
Ionescu, Cristina
Crăciun, Liviu
Sârbu, Ionela
author_facet Vamanu, Emanuel
Ene, Mihaela
Biță, Bogdan
Ionescu, Cristina
Crăciun, Liviu
Sârbu, Ionela
author_sort Vamanu, Emanuel
collection PubMed
description The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis process, using aqueous extract from Lactarius piperatus mushroom, and to characterize the nanomaterial. We determined its antimicrobial and antioxidant effects in vitro in the microbiota of healthy individuals via the GIS1 system—a colon transit simulator. Per the quantitative polymerase chain reaction (qPCR) results, the antimicrobial properties of the AgNPs affected the initial share of different enteric species by decreasing the Bacteroides, Enterobacteriaceae, and Lactobacillus populations and favoring the Bifidobacterium group. The association between AgNPs and wild mushroom L. piperatus extract had a synergistic antibacterial activity against various pathogenic microorganisms while the mushroom extract reduced biofilm formation. Administration of AgNP maintained its constant antioxidant status, and it was correlated with a reduction in ammonium compounds. The physicochemical characterization of these NPs complemented their biochemical characterization. The maximum ultraviolet-visible spectroscopy (UV-VIS) absorbance was observed at 440 nm, while the Fourier transform infrared (FT-IR) spectrum reached a peak at 3296 cm(–1), which was correlated with the high-performance liquid chromatographic analysis (HPLC). The major phenolic compound was homogentisic acid. The size (49 ± 16 nm in diameter) and spherical shape of the NPs were correlated with their biological effects in vitro.
format Online
Article
Text
id pubmed-5986487
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59864872018-06-05 In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract Vamanu, Emanuel Ene, Mihaela Biță, Bogdan Ionescu, Cristina Crăciun, Liviu Sârbu, Ionela Nutrients Article The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis process, using aqueous extract from Lactarius piperatus mushroom, and to characterize the nanomaterial. We determined its antimicrobial and antioxidant effects in vitro in the microbiota of healthy individuals via the GIS1 system—a colon transit simulator. Per the quantitative polymerase chain reaction (qPCR) results, the antimicrobial properties of the AgNPs affected the initial share of different enteric species by decreasing the Bacteroides, Enterobacteriaceae, and Lactobacillus populations and favoring the Bifidobacterium group. The association between AgNPs and wild mushroom L. piperatus extract had a synergistic antibacterial activity against various pathogenic microorganisms while the mushroom extract reduced biofilm formation. Administration of AgNP maintained its constant antioxidant status, and it was correlated with a reduction in ammonium compounds. The physicochemical characterization of these NPs complemented their biochemical characterization. The maximum ultraviolet-visible spectroscopy (UV-VIS) absorbance was observed at 440 nm, while the Fourier transform infrared (FT-IR) spectrum reached a peak at 3296 cm(–1), which was correlated with the high-performance liquid chromatographic analysis (HPLC). The major phenolic compound was homogentisic acid. The size (49 ± 16 nm in diameter) and spherical shape of the NPs were correlated with their biological effects in vitro. MDPI 2018-05-14 /pmc/articles/PMC5986487/ /pubmed/29757931 http://dx.doi.org/10.3390/nu10050607 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vamanu, Emanuel
Ene, Mihaela
Biță, Bogdan
Ionescu, Cristina
Crăciun, Liviu
Sârbu, Ionela
In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title_full In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title_fullStr In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title_full_unstemmed In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title_short In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract
title_sort in vitro human microbiota response to exposure to silver nanoparticles biosynthesized with mushroom extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986487/
https://www.ncbi.nlm.nih.gov/pubmed/29757931
http://dx.doi.org/10.3390/nu10050607
work_keys_str_mv AT vamanuemanuel invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract
AT enemihaela invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract
AT bitabogdan invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract
AT ionescucristina invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract
AT craciunliviu invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract
AT sarbuionela invitrohumanmicrobiotaresponsetoexposuretosilvernanoparticlesbiosynthesizedwithmushroomextract