Quantitative analysis of DNA with single-molecule sequencing

Cancer can be diagnosed by identifying DNA and microRNA base sequences that have the same base length yet differ in a few base sequences, if the abundance ratios of these slightly deviant base sequences can be determined. However, such quantitative analyses cannot be performed using the current DNA...

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Autores principales: Ohshiro, Takahito, Tsutsui, Makusu, Yokota, Kazumichi, Taniguchi, Masateru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986817/
https://www.ncbi.nlm.nih.gov/pubmed/29867186
http://dx.doi.org/10.1038/s41598-018-26875-7
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author Ohshiro, Takahito
Tsutsui, Makusu
Yokota, Kazumichi
Taniguchi, Masateru
author_facet Ohshiro, Takahito
Tsutsui, Makusu
Yokota, Kazumichi
Taniguchi, Masateru
author_sort Ohshiro, Takahito
collection PubMed
description Cancer can be diagnosed by identifying DNA and microRNA base sequences that have the same base length yet differ in a few base sequences, if the abundance ratios of these slightly deviant base sequences can be determined. However, such quantitative analyses cannot be performed using the current DNA sequencers. Here we determine entire base sequences of four types of DNA corresponding to the let-7 microRNA, which is a 22-base cancer marker. We record the single-molecule conductances of the base molecules using current-tunneling measurements. In addition, we count the numbers of molecules in a solution to determine the abundance ratios of two DNA strands that differ by a single base sequence.
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spelling pubmed-59868172018-06-07 Quantitative analysis of DNA with single-molecule sequencing Ohshiro, Takahito Tsutsui, Makusu Yokota, Kazumichi Taniguchi, Masateru Sci Rep Article Cancer can be diagnosed by identifying DNA and microRNA base sequences that have the same base length yet differ in a few base sequences, if the abundance ratios of these slightly deviant base sequences can be determined. However, such quantitative analyses cannot be performed using the current DNA sequencers. Here we determine entire base sequences of four types of DNA corresponding to the let-7 microRNA, which is a 22-base cancer marker. We record the single-molecule conductances of the base molecules using current-tunneling measurements. In addition, we count the numbers of molecules in a solution to determine the abundance ratios of two DNA strands that differ by a single base sequence. Nature Publishing Group UK 2018-06-04 /pmc/articles/PMC5986817/ /pubmed/29867186 http://dx.doi.org/10.1038/s41598-018-26875-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ohshiro, Takahito
Tsutsui, Makusu
Yokota, Kazumichi
Taniguchi, Masateru
Quantitative analysis of DNA with single-molecule sequencing
title Quantitative analysis of DNA with single-molecule sequencing
title_full Quantitative analysis of DNA with single-molecule sequencing
title_fullStr Quantitative analysis of DNA with single-molecule sequencing
title_full_unstemmed Quantitative analysis of DNA with single-molecule sequencing
title_short Quantitative analysis of DNA with single-molecule sequencing
title_sort quantitative analysis of dna with single-molecule sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986817/
https://www.ncbi.nlm.nih.gov/pubmed/29867186
http://dx.doi.org/10.1038/s41598-018-26875-7
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