A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern

The Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) has been shown to play a role in synaptic availability of glycine although the exact mechanism remains unclear. We used electrophysiological recordings and biochemical experiments to investigate the role of Asc-1 transporter in glycinergic t...

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Autores principales: Mesuret, Guillaume, Khabbazzadeh, Sepideh, Bischoff, Anne M., Safory, Hazem, Wolosker, Herman, Hülsmann, Swen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986860/
https://www.ncbi.nlm.nih.gov/pubmed/29867218
http://dx.doi.org/10.1038/s41598-018-26868-6
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author Mesuret, Guillaume
Khabbazzadeh, Sepideh
Bischoff, Anne M.
Safory, Hazem
Wolosker, Herman
Hülsmann, Swen
author_facet Mesuret, Guillaume
Khabbazzadeh, Sepideh
Bischoff, Anne M.
Safory, Hazem
Wolosker, Herman
Hülsmann, Swen
author_sort Mesuret, Guillaume
collection PubMed
description The Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) has been shown to play a role in synaptic availability of glycine although the exact mechanism remains unclear. We used electrophysiological recordings and biochemical experiments to investigate the role of Asc-1 transporter in glycinergic transmission in the brainstem respiratory network. Using both the Asc-1 substrate and transportable inhibitor D-isoleucine (D-Ile), and the non-transportable Asc-1 blocker Lu AE00527 (Lu), we found that D-Ile reduces glycinergic transmission and increases glycine release via hetero-exchange, whereas Lu has no acute effect on glycinergic synaptic transmission. Furthermore, D-Ile increases the frequency and reduces amplitude of the phrenic nerve activity in the arterially-perfused working heart brainstem preparation. These results suggest a role of Asc-1 in modulating presynaptic glycine levels that can impact on the respiratory network.
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spelling pubmed-59868602018-06-07 A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern Mesuret, Guillaume Khabbazzadeh, Sepideh Bischoff, Anne M. Safory, Hazem Wolosker, Herman Hülsmann, Swen Sci Rep Article The Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) has been shown to play a role in synaptic availability of glycine although the exact mechanism remains unclear. We used electrophysiological recordings and biochemical experiments to investigate the role of Asc-1 transporter in glycinergic transmission in the brainstem respiratory network. Using both the Asc-1 substrate and transportable inhibitor D-isoleucine (D-Ile), and the non-transportable Asc-1 blocker Lu AE00527 (Lu), we found that D-Ile reduces glycinergic transmission and increases glycine release via hetero-exchange, whereas Lu has no acute effect on glycinergic synaptic transmission. Furthermore, D-Ile increases the frequency and reduces amplitude of the phrenic nerve activity in the arterially-perfused working heart brainstem preparation. These results suggest a role of Asc-1 in modulating presynaptic glycine levels that can impact on the respiratory network. Nature Publishing Group UK 2018-06-04 /pmc/articles/PMC5986860/ /pubmed/29867218 http://dx.doi.org/10.1038/s41598-018-26868-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mesuret, Guillaume
Khabbazzadeh, Sepideh
Bischoff, Anne M.
Safory, Hazem
Wolosker, Herman
Hülsmann, Swen
A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title_full A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title_fullStr A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title_full_unstemmed A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title_short A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern
title_sort neuronal role of the alanine-serine-cysteine-1 transporter (slc7a10, asc-1) for glycine inhibitory transmission and respiratory pattern
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986860/
https://www.ncbi.nlm.nih.gov/pubmed/29867218
http://dx.doi.org/10.1038/s41598-018-26868-6
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