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Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5
CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986985/ https://www.ncbi.nlm.nih.gov/pubmed/29872736 http://dx.doi.org/10.1016/j.bbrep.2018.03.007 |
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author | Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Kaneko, Mika K. Kato, Yukinari |
author_facet | Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Kaneko, Mika K. Kato, Yukinari |
author_sort | Yamada, Shinji |
collection | PubMed |
description | CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (CD44s), which lacks variant exons, is widely expressed on the surface of most tissues and all hematopoietic cells. In contrast, CD44 variant isoforms show tissue-specific expression patterns and have been extensively studied as both prognostic markers and therapeutic targets in cancer and other diseases. In this study, we immunized mice with CHO-K1 cell lines overexpressing CD44v3-10 to obtain novel anti-CD44 mAbs. One of the clones, C(44)Mab-5 (IgG(1), kappa), recognized both CD44s and CD44v3-10. C(44)Mab-5 also reacted with oral cancer cells such as Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4 using flow cytometry. Moreover, immunohistochemical analysis revealed that C(44)Mab-5 detected 166/182 (91.2%) of oral cancers. These results suggest that the C(44)Mab-5 antibody may be useful for investigating the expression and function of CD44 in various cancers. |
format | Online Article Text |
id | pubmed-5986985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-59869852018-06-05 Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Kaneko, Mika K. Kato, Yukinari Biochem Biophys Rep Research Article CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (CD44s), which lacks variant exons, is widely expressed on the surface of most tissues and all hematopoietic cells. In contrast, CD44 variant isoforms show tissue-specific expression patterns and have been extensively studied as both prognostic markers and therapeutic targets in cancer and other diseases. In this study, we immunized mice with CHO-K1 cell lines overexpressing CD44v3-10 to obtain novel anti-CD44 mAbs. One of the clones, C(44)Mab-5 (IgG(1), kappa), recognized both CD44s and CD44v3-10. C(44)Mab-5 also reacted with oral cancer cells such as Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4 using flow cytometry. Moreover, immunohistochemical analysis revealed that C(44)Mab-5 detected 166/182 (91.2%) of oral cancers. These results suggest that the C(44)Mab-5 antibody may be useful for investigating the expression and function of CD44 in various cancers. Elsevier 2018-04-12 /pmc/articles/PMC5986985/ /pubmed/29872736 http://dx.doi.org/10.1016/j.bbrep.2018.03.007 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Kaneko, Mika K. Kato, Yukinari Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title | Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title_full | Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title_fullStr | Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title_full_unstemmed | Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title_short | Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C(44)Mab-5 |
title_sort | detection of high cd44 expression in oral cancers using the novel monoclonal antibody, c(44)mab-5 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986985/ https://www.ncbi.nlm.nih.gov/pubmed/29872736 http://dx.doi.org/10.1016/j.bbrep.2018.03.007 |
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