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New insights about the regulation of Nidovirus subgenomic mRNA synthesis

The members of the Order Nidovirales share a similar genome organization with two overlapping nonstructural polyproteins encoded in the 5′ two-thirds and the structural proteins encoded in the 3′ third. They also express their 3′ region proteins from a nested set of 3′ co-terminal subgenomic messeng...

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Detalles Bibliográficos
Autores principales: Di, Han, McIntyre, Ayisha A., Brinton, Margo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987246/
https://www.ncbi.nlm.nih.gov/pubmed/29475599
http://dx.doi.org/10.1016/j.virol.2018.01.026
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author Di, Han
McIntyre, Ayisha A.
Brinton, Margo A.
author_facet Di, Han
McIntyre, Ayisha A.
Brinton, Margo A.
author_sort Di, Han
collection PubMed
description The members of the Order Nidovirales share a similar genome organization with two overlapping nonstructural polyproteins encoded in the 5′ two-thirds and the structural proteins encoded in the 3′ third. They also express their 3′ region proteins from a nested set of 3′ co-terminal subgenomic messenger RNAs (sg mRNAs). Some but not all of the Nidovirus sg mRNAs also have a common 5′ leader sequence that is acquired by a discontinuous RNA synthesis mechanism regulated by multiple 3′ body transcription regulating sequences (TRSs) and the 5′ leader TRS. Initial studies detected a single major body TRS for each 3′ sg mRNA with a few alternative functional TRSs reported. The recent application of advanced techniques, such as next generation sequencing and ribosomal profiling, in studies of arteriviruses and coronaviruses has revealed an expanded sg mRNA transcriptome and coding capacity.
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spelling pubmed-59872462019-04-01 New insights about the regulation of Nidovirus subgenomic mRNA synthesis Di, Han McIntyre, Ayisha A. Brinton, Margo A. Virology Article The members of the Order Nidovirales share a similar genome organization with two overlapping nonstructural polyproteins encoded in the 5′ two-thirds and the structural proteins encoded in the 3′ third. They also express their 3′ region proteins from a nested set of 3′ co-terminal subgenomic messenger RNAs (sg mRNAs). Some but not all of the Nidovirus sg mRNAs also have a common 5′ leader sequence that is acquired by a discontinuous RNA synthesis mechanism regulated by multiple 3′ body transcription regulating sequences (TRSs) and the 5′ leader TRS. Initial studies detected a single major body TRS for each 3′ sg mRNA with a few alternative functional TRSs reported. The recent application of advanced techniques, such as next generation sequencing and ribosomal profiling, in studies of arteriviruses and coronaviruses has revealed an expanded sg mRNA transcriptome and coding capacity. Elsevier Inc. 2018-04 2018-02-21 /pmc/articles/PMC5987246/ /pubmed/29475599 http://dx.doi.org/10.1016/j.virol.2018.01.026 Text en © 2018 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Di, Han
McIntyre, Ayisha A.
Brinton, Margo A.
New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title_full New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title_fullStr New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title_full_unstemmed New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title_short New insights about the regulation of Nidovirus subgenomic mRNA synthesis
title_sort new insights about the regulation of nidovirus subgenomic mrna synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987246/
https://www.ncbi.nlm.nih.gov/pubmed/29475599
http://dx.doi.org/10.1016/j.virol.2018.01.026
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