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Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears

Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and spa...

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Autores principales: Ogunsola, John, Antia, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987350/
https://www.ncbi.nlm.nih.gov/pubmed/29911022
http://dx.doi.org/10.4314/ovj.v8i2.11
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author Ogunsola, John
Antia, Richard
author_facet Ogunsola, John
Antia, Richard
author_sort Ogunsola, John
collection PubMed
description Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and sparsely been used in human and veterinary histopathology respectively. However, two major drawbacks have been irreproducible results and excessive staining precipitates. This study seeks to adapt the technique to canine peripheral blood smears in order to establish a routine staining protocol. Standardized volumes and concentrations of silver nitrate, gelatin, and formic acid were applied to smears at different temperatures for varying lengths of time. The technique was applied to unfixed and fixed smears. In some cases, a reducing agent (1% potassium iodide) was applied. It was shown that the optimum staining protocol was achieved by applying standardized solutions to a fixed smear at 46°C for 50 minutes. It is concluded that the staining protocol outlined in this study is practicable, and produces excellent and reproducible results that would enhance evaluation of AgNORs in canine peripheral blood cells.
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spelling pubmed-59873502018-06-15 Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears Ogunsola, John Antia, Richard Open Vet J Short Communication Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and sparsely been used in human and veterinary histopathology respectively. However, two major drawbacks have been irreproducible results and excessive staining precipitates. This study seeks to adapt the technique to canine peripheral blood smears in order to establish a routine staining protocol. Standardized volumes and concentrations of silver nitrate, gelatin, and formic acid were applied to smears at different temperatures for varying lengths of time. The technique was applied to unfixed and fixed smears. In some cases, a reducing agent (1% potassium iodide) was applied. It was shown that the optimum staining protocol was achieved by applying standardized solutions to a fixed smear at 46°C for 50 minutes. It is concluded that the staining protocol outlined in this study is practicable, and produces excellent and reproducible results that would enhance evaluation of AgNORs in canine peripheral blood cells. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018 2018-05-25 /pmc/articles/PMC5987350/ /pubmed/29911022 http://dx.doi.org/10.4314/ovj.v8i2.11 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Short Communication
Ogunsola, John
Antia, Richard
Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title_full Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title_fullStr Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title_full_unstemmed Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title_short Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
title_sort adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987350/
https://www.ncbi.nlm.nih.gov/pubmed/29911022
http://dx.doi.org/10.4314/ovj.v8i2.11
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