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Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears
Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and spa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987350/ https://www.ncbi.nlm.nih.gov/pubmed/29911022 http://dx.doi.org/10.4314/ovj.v8i2.11 |
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author | Ogunsola, John Antia, Richard |
author_facet | Ogunsola, John Antia, Richard |
author_sort | Ogunsola, John |
collection | PubMed |
description | Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and sparsely been used in human and veterinary histopathology respectively. However, two major drawbacks have been irreproducible results and excessive staining precipitates. This study seeks to adapt the technique to canine peripheral blood smears in order to establish a routine staining protocol. Standardized volumes and concentrations of silver nitrate, gelatin, and formic acid were applied to smears at different temperatures for varying lengths of time. The technique was applied to unfixed and fixed smears. In some cases, a reducing agent (1% potassium iodide) was applied. It was shown that the optimum staining protocol was achieved by applying standardized solutions to a fixed smear at 46°C for 50 minutes. It is concluded that the staining protocol outlined in this study is practicable, and produces excellent and reproducible results that would enhance evaluation of AgNORs in canine peripheral blood cells. |
format | Online Article Text |
id | pubmed-5987350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59873502018-06-15 Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears Ogunsola, John Antia, Richard Open Vet J Short Communication Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and sparsely been used in human and veterinary histopathology respectively. However, two major drawbacks have been irreproducible results and excessive staining precipitates. This study seeks to adapt the technique to canine peripheral blood smears in order to establish a routine staining protocol. Standardized volumes and concentrations of silver nitrate, gelatin, and formic acid were applied to smears at different temperatures for varying lengths of time. The technique was applied to unfixed and fixed smears. In some cases, a reducing agent (1% potassium iodide) was applied. It was shown that the optimum staining protocol was achieved by applying standardized solutions to a fixed smear at 46°C for 50 minutes. It is concluded that the staining protocol outlined in this study is practicable, and produces excellent and reproducible results that would enhance evaluation of AgNORs in canine peripheral blood cells. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018 2018-05-25 /pmc/articles/PMC5987350/ /pubmed/29911022 http://dx.doi.org/10.4314/ovj.v8i2.11 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Short Communication Ogunsola, John Antia, Richard Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title | Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title_full | Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title_fullStr | Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title_full_unstemmed | Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title_short | Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
title_sort | adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987350/ https://www.ncbi.nlm.nih.gov/pubmed/29911022 http://dx.doi.org/10.4314/ovj.v8i2.11 |
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