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Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato

BACKGROUND: BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the defense response mechanism underlying this BcGs1 elicitation in...

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Autores principales: Yang, Chenyu, Liang, Yingbo, Qiu, Dewen, Zeng, Hongmei, Yuan, Jingjing, Yang, Xiufen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987389/
https://www.ncbi.nlm.nih.gov/pubmed/29866036
http://dx.doi.org/10.1186/s12870-018-1319-0
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author Yang, Chenyu
Liang, Yingbo
Qiu, Dewen
Zeng, Hongmei
Yuan, Jingjing
Yang, Xiufen
author_facet Yang, Chenyu
Liang, Yingbo
Qiu, Dewen
Zeng, Hongmei
Yuan, Jingjing
Yang, Xiufen
author_sort Yang, Chenyu
collection PubMed
description BACKGROUND: BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the defense response mechanism underlying this BcGs1 elicitation in tomato. RESULTS: We revealed that the two domains were required for BcGs1’s full necrosis activity. According to analysis and quantitative real-time PCR of the up-regulated proteins and genes filtered by iTRAQ-based quantitative proteome approach, oxidative metabolism and phenylpropanoid metabolism were speculated to be involved in BcGs1-triggered defense response in tomato. Furthermore, experimental evidence showed that BcGs1 triggered reactive oxygen species (ROS) burst and increased the level of phenylalanine-ammonia lyase (PAL) and peroxidase (POD) enzyme activity, as well as lignin accumulation. Moreover, histochemical analysis revealed that infiltration of BcGs1 in tomato leaves exhibited cell wall thickening compared with untreated plants. CONCLUSIONS: The results suggested that BcGs1 activated the basal defense response included lignin metabolism contributed to BcGs1-induced resistance to Botrytis. cinerea infection in tomato. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1319-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-59873892018-07-10 Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato Yang, Chenyu Liang, Yingbo Qiu, Dewen Zeng, Hongmei Yuan, Jingjing Yang, Xiufen BMC Plant Biol Research Article BACKGROUND: BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the defense response mechanism underlying this BcGs1 elicitation in tomato. RESULTS: We revealed that the two domains were required for BcGs1’s full necrosis activity. According to analysis and quantitative real-time PCR of the up-regulated proteins and genes filtered by iTRAQ-based quantitative proteome approach, oxidative metabolism and phenylpropanoid metabolism were speculated to be involved in BcGs1-triggered defense response in tomato. Furthermore, experimental evidence showed that BcGs1 triggered reactive oxygen species (ROS) burst and increased the level of phenylalanine-ammonia lyase (PAL) and peroxidase (POD) enzyme activity, as well as lignin accumulation. Moreover, histochemical analysis revealed that infiltration of BcGs1 in tomato leaves exhibited cell wall thickening compared with untreated plants. CONCLUSIONS: The results suggested that BcGs1 activated the basal defense response included lignin metabolism contributed to BcGs1-induced resistance to Botrytis. cinerea infection in tomato. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1319-0) contains supplementary material, which is available to authorized users. BioMed Central 2018-06-04 /pmc/articles/PMC5987389/ /pubmed/29866036 http://dx.doi.org/10.1186/s12870-018-1319-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Chenyu
Liang, Yingbo
Qiu, Dewen
Zeng, Hongmei
Yuan, Jingjing
Yang, Xiufen
Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title_full Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title_fullStr Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title_full_unstemmed Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title_short Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato
title_sort lignin metabolism involves botrytis cinerea bcgs1- induced defense response in tomato
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987389/
https://www.ncbi.nlm.nih.gov/pubmed/29866036
http://dx.doi.org/10.1186/s12870-018-1319-0
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