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Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles
BACKGROUND: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. RESULTS: It was found that under symmetrical 100 mM K(+)...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987474/ https://www.ncbi.nlm.nih.gov/pubmed/29866031 http://dx.doi.org/10.1186/s12870-018-1321-6 |
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author | Burdach, Zbigniew Siemieniuk, Agnieszka Trela, Zenon Kurtyka, Renata Karcz, Waldemar |
author_facet | Burdach, Zbigniew Siemieniuk, Agnieszka Trela, Zenon Kurtyka, Renata Karcz, Waldemar |
author_sort | Burdach, Zbigniew |
collection | PubMed |
description | BACKGROUND: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. RESULTS: It was found that under symmetrical 100 mM K(+) and 100 μM cytoplasmic Ca(2+) the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, τ, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. CONCLUSIONS: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA). |
format | Online Article Text |
id | pubmed-5987474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59874742018-07-10 Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles Burdach, Zbigniew Siemieniuk, Agnieszka Trela, Zenon Kurtyka, Renata Karcz, Waldemar BMC Plant Biol Research Article BACKGROUND: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. RESULTS: It was found that under symmetrical 100 mM K(+) and 100 μM cytoplasmic Ca(2+) the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, τ, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. CONCLUSIONS: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA). BioMed Central 2018-06-04 /pmc/articles/PMC5987474/ /pubmed/29866031 http://dx.doi.org/10.1186/s12870-018-1321-6 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Burdach, Zbigniew Siemieniuk, Agnieszka Trela, Zenon Kurtyka, Renata Karcz, Waldemar Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title | Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title_full | Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title_fullStr | Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title_full_unstemmed | Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title_short | Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles |
title_sort | role of auxin (iaa) in the regulation of slow vacuolar (sv) channels and the volume of red beet taproot vacuoles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987474/ https://www.ncbi.nlm.nih.gov/pubmed/29866031 http://dx.doi.org/10.1186/s12870-018-1321-6 |
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