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Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada

BACKGROUND: In September 2009, a mumps outbreak originated in New York and spread to Northeastern USA and Canada. This study compares the performance of different diagnostic testing methods used in Ontario and describes molecular characteristics of the outbreak strain. METHODS: Between September 200...

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Autores principales: L’Huillier, Arnaud G., Eshaghi, Alireza, Racey, C. Sarai, Ogbulafor, Katherene, Lombos, Ernesto, Higgins, Rachel R., Alexander, David C., Kristjanson, Erik, Maregmen, Jocelyn, Gubbay, Jonathan B., Mazzulli, Tony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987625/
https://www.ncbi.nlm.nih.gov/pubmed/29866178
http://dx.doi.org/10.1186/s12985-018-0996-5
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author L’Huillier, Arnaud G.
Eshaghi, Alireza
Racey, C. Sarai
Ogbulafor, Katherene
Lombos, Ernesto
Higgins, Rachel R.
Alexander, David C.
Kristjanson, Erik
Maregmen, Jocelyn
Gubbay, Jonathan B.
Mazzulli, Tony
author_facet L’Huillier, Arnaud G.
Eshaghi, Alireza
Racey, C. Sarai
Ogbulafor, Katherene
Lombos, Ernesto
Higgins, Rachel R.
Alexander, David C.
Kristjanson, Erik
Maregmen, Jocelyn
Gubbay, Jonathan B.
Mazzulli, Tony
author_sort L’Huillier, Arnaud G.
collection PubMed
description BACKGROUND: In September 2009, a mumps outbreak originated in New York and spread to Northeastern USA and Canada. This study compares the performance of different diagnostic testing methods used in Ontario and describes molecular characteristics of the outbreak strain. METHODS: Between September 2009 and February 2010, specimens from suspect cases were submitted to Public Health Ontario Laboratory for mumps serology, culture and/or real-time reverse-transcriptase PCR (rRT-PCR) testing. rRT-PCR-positive specimens underwent genotyping at Canada’s National Microbiology Laboratory. Whole genome sequencing was performed on four outbreak and three sporadic viral culture isolates. RESULTS: Six hundred ninety-eight patients had IgM serology testing, of which 255 (37%) had culture and rRT-PCR. Among those, 35/698 (5%) were IgM positive, 39/255 (15%) culture positive and 47/255 (18%) rRT-PCR-positive. Buccal swabs had the highest rRT-PCR positivity (21%). The outbreak isolates were identical to that in the New York outbreak occurring at the same time. Nucleotide and amino acid identity with the Jeryl Lynn vaccine strain ranged from 85.0-94.5% and 82.4-99.4%, depending on the gene and coding sequences. Homology of the HN protein, the main immunogenic mumps virus protein, was found to be 94.5 and 95.3%, when compared to Jeryl Lynn vaccine major and minor components, respectively. CONCLUSIONS: Despite higher sensitivity than serology, rRT-PCR testing is underutilized. Further work is needed to better understand the suboptimal match of the HN gene between the outbreak strain and the Jeryl Lynn vaccine strain.
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spelling pubmed-59876252018-06-20 Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada L’Huillier, Arnaud G. Eshaghi, Alireza Racey, C. Sarai Ogbulafor, Katherene Lombos, Ernesto Higgins, Rachel R. Alexander, David C. Kristjanson, Erik Maregmen, Jocelyn Gubbay, Jonathan B. Mazzulli, Tony Virol J Research BACKGROUND: In September 2009, a mumps outbreak originated in New York and spread to Northeastern USA and Canada. This study compares the performance of different diagnostic testing methods used in Ontario and describes molecular characteristics of the outbreak strain. METHODS: Between September 2009 and February 2010, specimens from suspect cases were submitted to Public Health Ontario Laboratory for mumps serology, culture and/or real-time reverse-transcriptase PCR (rRT-PCR) testing. rRT-PCR-positive specimens underwent genotyping at Canada’s National Microbiology Laboratory. Whole genome sequencing was performed on four outbreak and three sporadic viral culture isolates. RESULTS: Six hundred ninety-eight patients had IgM serology testing, of which 255 (37%) had culture and rRT-PCR. Among those, 35/698 (5%) were IgM positive, 39/255 (15%) culture positive and 47/255 (18%) rRT-PCR-positive. Buccal swabs had the highest rRT-PCR positivity (21%). The outbreak isolates were identical to that in the New York outbreak occurring at the same time. Nucleotide and amino acid identity with the Jeryl Lynn vaccine strain ranged from 85.0-94.5% and 82.4-99.4%, depending on the gene and coding sequences. Homology of the HN protein, the main immunogenic mumps virus protein, was found to be 94.5 and 95.3%, when compared to Jeryl Lynn vaccine major and minor components, respectively. CONCLUSIONS: Despite higher sensitivity than serology, rRT-PCR testing is underutilized. Further work is needed to better understand the suboptimal match of the HN gene between the outbreak strain and the Jeryl Lynn vaccine strain. BioMed Central 2018-06-05 /pmc/articles/PMC5987625/ /pubmed/29866178 http://dx.doi.org/10.1186/s12985-018-0996-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
L’Huillier, Arnaud G.
Eshaghi, Alireza
Racey, C. Sarai
Ogbulafor, Katherene
Lombos, Ernesto
Higgins, Rachel R.
Alexander, David C.
Kristjanson, Erik
Maregmen, Jocelyn
Gubbay, Jonathan B.
Mazzulli, Tony
Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title_full Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title_fullStr Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title_full_unstemmed Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title_short Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada
title_sort laboratory testing and phylogenetic analysis during a mumps outbreak in ontario, canada
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987625/
https://www.ncbi.nlm.nih.gov/pubmed/29866178
http://dx.doi.org/10.1186/s12985-018-0996-5
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