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TRRAP is a central regulator of human multiciliated cell formation
The multiciliated cell (MCC) is an evolutionarily conserved cell type, which in vertebrates functions to promote directional fluid flow across epithelial tissues. In the conducting airway, MCCs are generated by basal stem/progenitor cells and act in concert with secretory cells to perform mucociliar...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987713/ https://www.ncbi.nlm.nih.gov/pubmed/29588376 http://dx.doi.org/10.1083/jcb.201706106 |
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author | Wang, Zhao Plasschaert, Lindsey W. Aryal, Shivani Renaud, Nicole A. Yang, Zinger Choo-Wing, Rayman Pessotti, Angelica D. Kirkpatrick, Nathaniel D. Cochran, Nadire R. Carbone, Walter Maher, Rob Lindeman, Alicia Russ, Carsten Reece-Hoyes, John McAllister, Gregory Hoffman, Gregory R. Roma, Guglielmo Jaffe, Aron B. |
author_facet | Wang, Zhao Plasschaert, Lindsey W. Aryal, Shivani Renaud, Nicole A. Yang, Zinger Choo-Wing, Rayman Pessotti, Angelica D. Kirkpatrick, Nathaniel D. Cochran, Nadire R. Carbone, Walter Maher, Rob Lindeman, Alicia Russ, Carsten Reece-Hoyes, John McAllister, Gregory Hoffman, Gregory R. Roma, Guglielmo Jaffe, Aron B. |
author_sort | Wang, Zhao |
collection | PubMed |
description | The multiciliated cell (MCC) is an evolutionarily conserved cell type, which in vertebrates functions to promote directional fluid flow across epithelial tissues. In the conducting airway, MCCs are generated by basal stem/progenitor cells and act in concert with secretory cells to perform mucociliary clearance to expel pathogens from the lung. Studies in multiple systems, including Xenopus laevis epidermis, murine trachea, and zebrafish kidney, have uncovered a transcriptional network that regulates multiple steps of multiciliogenesis, ultimately leading to an MCC with hundreds of motile cilia extended from their apical surface, which beat in a coordinated fashion. Here, we used a pool-based short hairpin RNA screening approach and identified TRRAP, an essential component of multiple histone acetyltransferase complexes, as a central regulator of MCC formation. Using a combination of immunofluorescence, signaling pathway modulation, and genomic approaches, we show that (a) TRRAP acts downstream of the Notch2-mediated basal progenitor cell fate decision and upstream of Multicilin to control MCC differentiation; and (b) TRRAP binds to the promoters and regulates the expression of a network of genes involved in MCC differentiation and function, including several genes associated with human ciliopathies. |
format | Online Article Text |
id | pubmed-5987713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59877132018-06-07 TRRAP is a central regulator of human multiciliated cell formation Wang, Zhao Plasschaert, Lindsey W. Aryal, Shivani Renaud, Nicole A. Yang, Zinger Choo-Wing, Rayman Pessotti, Angelica D. Kirkpatrick, Nathaniel D. Cochran, Nadire R. Carbone, Walter Maher, Rob Lindeman, Alicia Russ, Carsten Reece-Hoyes, John McAllister, Gregory Hoffman, Gregory R. Roma, Guglielmo Jaffe, Aron B. J Cell Biol Research Articles The multiciliated cell (MCC) is an evolutionarily conserved cell type, which in vertebrates functions to promote directional fluid flow across epithelial tissues. In the conducting airway, MCCs are generated by basal stem/progenitor cells and act in concert with secretory cells to perform mucociliary clearance to expel pathogens from the lung. Studies in multiple systems, including Xenopus laevis epidermis, murine trachea, and zebrafish kidney, have uncovered a transcriptional network that regulates multiple steps of multiciliogenesis, ultimately leading to an MCC with hundreds of motile cilia extended from their apical surface, which beat in a coordinated fashion. Here, we used a pool-based short hairpin RNA screening approach and identified TRRAP, an essential component of multiple histone acetyltransferase complexes, as a central regulator of MCC formation. Using a combination of immunofluorescence, signaling pathway modulation, and genomic approaches, we show that (a) TRRAP acts downstream of the Notch2-mediated basal progenitor cell fate decision and upstream of Multicilin to control MCC differentiation; and (b) TRRAP binds to the promoters and regulates the expression of a network of genes involved in MCC differentiation and function, including several genes associated with human ciliopathies. Rockefeller University Press 2018-06-04 /pmc/articles/PMC5987713/ /pubmed/29588376 http://dx.doi.org/10.1083/jcb.201706106 Text en © 2018 Wang et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Wang, Zhao Plasschaert, Lindsey W. Aryal, Shivani Renaud, Nicole A. Yang, Zinger Choo-Wing, Rayman Pessotti, Angelica D. Kirkpatrick, Nathaniel D. Cochran, Nadire R. Carbone, Walter Maher, Rob Lindeman, Alicia Russ, Carsten Reece-Hoyes, John McAllister, Gregory Hoffman, Gregory R. Roma, Guglielmo Jaffe, Aron B. TRRAP is a central regulator of human multiciliated cell formation |
title | TRRAP is a central regulator of human multiciliated cell formation |
title_full | TRRAP is a central regulator of human multiciliated cell formation |
title_fullStr | TRRAP is a central regulator of human multiciliated cell formation |
title_full_unstemmed | TRRAP is a central regulator of human multiciliated cell formation |
title_short | TRRAP is a central regulator of human multiciliated cell formation |
title_sort | trrap is a central regulator of human multiciliated cell formation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987713/ https://www.ncbi.nlm.nih.gov/pubmed/29588376 http://dx.doi.org/10.1083/jcb.201706106 |
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